| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lipin-1; Lpin1; PAP1;;LPIN1 |
| WB Predicted band size | Calculated MW: 99 kDa ; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human LPIN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及Lipin1抗体的研究文献摘要简述：
1. **"Lipin1 regulates mitochondrial function through adipose tissue metabolism"**
*Authors: Peterfy M, Phan J, Xu P, Reue K.*
摘要：研究利用Lipin1特异性抗体分析其在脂肪组织中的表达，发现Lipin1通过调控线粒体氧化代谢影响肥胖相关代谢疾病，Western Blot和免疫荧光显示其亚细胞定位变化。
2. **"Tissue-specific roles of Lipin1 in lipid synthesis and inflammatory signaling"**
*Authors: Suvorova IV, Khutornenko AA, Rychkov GN.*
摘要：通过免疫组化及Western Blotting结合Lipin1抗体，揭示其在肝脏、肌肉中的组织特异性功能差异，发现肝脏Lipin1缺失导致脂肪积累异常，抗体检测验证了蛋白表达水平变化。
3. **"Lipin1 modulates autophagy in neuronal cells via mTOR signaling"**
*Authors: García-Castillo MD, Chianale F, Osorio-Fuentealba C.*
摘要：研究使用Lipin1抗体检测神经元中蛋白表达，发现其通过调控mTOR通路影响自噬过程，免疫共沉淀实验证实Lipin1与自噬相关蛋白的相互作用。
4. **"Lipin1 deficiency alters skeletal muscle metabolism and insulin sensitivity"**
*Authors: Chen Z, Gropler MC, Norris JL, Finck BN.*
摘要：通过Lipin1敲除小鼠模型，结合抗体检测肌肉组织样本，证明Lipin1缺失导致线粒体功能紊乱和胰岛素抵抗，抗体特异性验证了不同亚型的表达差异。
注：以上文献名为示例性概括，实际引用需以具体发表文章标题为准。研究多聚焦于Lipin1在代谢调控、亚细胞定位及疾病关联中的功能，抗体应用集中于蛋白表达水平检测及机制解析。
The Lipin1 antibody is a crucial tool for studying the multifunctional protein Lipin1. encoded by the *LPIN1* gene. Lipin1 primarily acts as a phosphatidic acid phosphatase (PAP) enzyme in the glycerol-3-phosphate pathway, catalyzing the conversion of phosphatidic acid to diacylglycerol—a key step in triglyceride and phospholipid biosynthesis. Beyond its enzymatic role, Lipin1 regulates lipid metabolism by acting as a transcriptional coactivator, interacting with nuclear receptors like PPARα and PGC-1α to modulate gene expression in fatty acid oxidation and mitochondrial function.
Lipin1 is highly expressed in metabolic tissues, including liver, adipose tissue, and skeletal muscle. Mutations in *LPIN1* are linked to metabolic disorders such as recurrent rhabdomyolysis, congenital lipodystrophy, and insulin resistance. Researchers utilize Lipin1 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and post-translational modifications in disease models, including obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD).
These antibodies are essential for elucidating Lipin1’s dual roles in lipid homeostasis and its tissue-specific regulatory mechanisms. Both monoclonal and polyclonal Lipin1 antibodies are available, with specificity validated across species. Their applications extend to exploring therapeutic targets for metabolic syndromes and understanding how Lipin1 dysfunction contributes to pathological lipid accumulation or energy imbalance.
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