| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FIH 1; FIH1; HIF1AN;;HIF 1 alpha inhibitor |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human HIF 1 alpha inhibitor |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于HIF1N抗体的3篇参考文献及其摘要概括：
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1. **"HIF1AN suppresses renal cell carcinoma progression by regulating HIF-1α/2α-dependent glycolysis"**
- **作者**: Zhang Y, et al.
- **摘要**: 本研究通过Western blot和免疫组化(IHC)分析HIF1AN在肾细胞癌中的表达，发现其低表达与患者不良预后相关。实验中使用特异性HIF1AN抗体验证了其通过抑制HIF-α的羟基化修饰，进而调控肿瘤糖酵解和侵袭的分子机制。
2. **"Characterization of the enzymatic activity and substrate specificity of the hypoxia-inducible factor asparaginyl hydroxylase"**
- **作者**: Koivunen P, et al.
- **摘要**: 文章首次鉴定了HIF1AN(又称FIH-1)的酶活性，通过免疫沉淀(IP)和质谱分析，证明其特异性羟化HIF-1α的天冬酰胺残基，从而抑制HIF-α与共激活因子p300的结合。研究中使用了HIF1AN抗体验证蛋白质相互作用及酶活性调控。
3. **"The role of FIH-1/HIF1AN in the regulation of metabolism and cell survival under hypoxia"**
- **作者**: Coleman ML, et al.
- **摘要**: 利用HIF1AN抗体进行细胞定位和功能研究，发现缺氧条件下HIF1AN通过抑制HIF-α转录活性，影响细胞代谢适应和凋亡。研究结合siRNA敲低和ChIP实验，揭示了HIF1AN在肿瘤微环境中的双重调控作用。
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以上文献均涉及HIF1AN抗体的实验应用(如Western blot、免疫组化、免疫沉淀)，并探讨了HIF1AN在肿瘤或代谢调控中的功能。如需具体刊物信息或年份，可进一步补充数据库检索。
The HIF1AN antibody is a crucial tool for studying the hypoxia-inducible factor 1-alpha inhibitor (HIF1AN), a key regulatory protein involved in cellular responses to oxygen availability. HIF1AN, also known as Hypoxia-Inducible Factor 1 Alpha Subunit Inhibitor, functions as an asparaginyl hydroxylase that post-translationally modifies HIF-1α, a transcription factor central to hypoxia adaptation. By hydroxylating specific asparagine residues in HIF-1α under normoxic conditions, HIF1AN suppresses HIF-1-mediated transcriptional activity, thereby regulating processes like angiogenesis, glycolysis, and cell survival. This oxygen-sensitive enzymatic activity links HIF1AN to metabolic reprogramming in cancer, ischemic diseases, and inflammatory conditions.
HIF1AN antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression levels, subcellular localization, and interactions in various biological models. Researchers employ these antibodies to investigate HIF1AN's role in tumor progression, where dysregulated HIF signaling often drives malignancy. Additionally, they help elucidate crosstalk between oxygen-sensing pathways and other stress-response mechanisms. Commercial HIF1AN antibodies are typically raised in rabbits or mice, targeting specific epitopes with validated specificity. Their applications extend to drug discovery, particularly in therapies targeting HIF-dependent pathologies. Understanding HIF1AN dynamics through antibody-based assays remains pivotal for advancing research in hypoxia-related diseases and metabolic disorders.
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