| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAP70; CLAMP; PDZD1; NHERF3; NHERF-3;;PDZK1 |
| WB Predicted band size | Calculated MW: 57 kDa ; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PDZK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于PDZK1抗体的参考文献及其摘要概括：
1. **文献名称**：*"PDZK1. a novel ERM-like domain-containing protein regulating the expression and localization of the scavenger receptor SR-BI"*
**作者**：Kocher O, et al.
**摘要**：该研究利用PDZK1抗体通过免疫沉淀和免疫荧光技术，揭示了PDZK1通过与SR-BI受体结合调控其在肝细胞膜上的定位及胆固醇代谢功能。
2. **文献名称**：*"Interaction of the PDZ1 domain of NHERF1 and the cytoplasmic tail of the CFTR chloride channel"*
**作者**：Gisler SM, et al.
**摘要**：通过Western blot和免疫共沉淀实验(使用PDZK1抗体)，研究发现PDZK1与囊性纤维化跨膜传导调节因子(CFTR)相互作用，影响氯离子通道的稳定性与功能。
3. **文献名称**：*"Downregulation of PDZK1 expression in human colorectal cancer cells promotes tumor progression"*
**作者**：Wang P, et al.
**摘要**：该文献利用PDZK1抗体检测结直肠癌细胞中的蛋白表达，发现PDZK1表达下调与肿瘤侵袭性增强相关，提示其作为潜在抑癌基因的作用。
4. **文献名称**：*"PDZK1 regulates intestinal transporter activity and membrane localization through scaffolding interactions"*
**作者**：Ito K, et al.
**摘要**：通过免疫组化和免疫印迹(使用PDZK1抗体)，研究证明PDZK1通过支架作用调控肠道转运蛋白(如PEPT1)的膜定位及功能，影响药物吸收与代谢。
这些文献均通过PDZK1抗体开展实验，聚焦于其在代谢调控、癌症及膜蛋白互作中的生物学功能。
The PDZK1 antibody is a crucial tool for investigating the PDZK1 protein, a scaffold protein involved in organizing multi-protein complexes and regulating cellular signaling pathways. PDZK1 (Post-synaptic density protein 95/Discs large/Zonula occludens-1 [PDZ] domain-containing protein 1) contains four PDZ domains, enabling it to interact with diverse membrane proteins, including receptors, transporters, and ion channels. It plays a key role in maintaining cellular homeostasis, particularly in tissues such as the liver, kidney, and intestine. For example, PDZK1 modulates the activity of scavenger receptor SR-BI (in cholesterol metabolism) and CFTR (cystic fibrosis transmembrane conductance regulator), impacting lipid absorption and ion transport.
PDZK1 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Dysregulation of PDZK1 has been linked to diseases such as cancer (e.g., breast, colon, and hepatocellular carcinoma), cardiovascular disorders, and metabolic syndromes. Studies suggest PDZK1 may act as a tumor suppressor or oncogene depending on context, influencing cell proliferation, apoptosis, and metastasis. Its role in drug resistance and response to therapies (e.g., chemotherapeutic agents) is also under investigation.
These antibodies are essential for elucidating PDZK1's molecular mechanisms and its potential as a diagnostic or therapeutic target in precision medicine.
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