| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIS1; LIS2; MDCR; MDS; PAFAH alpha; PAFAH; PAFAH1B1; PAFAHA;;LIS1 |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human LIS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与LIS1抗体相关的文献摘要概括：
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1. **文献名称**: *"LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome"*
**作者**: Reiner O. et al.
**摘要**: 研究通过免疫共沉淀和Western blot技术，发现LIS1蛋白通过与中心体组分mNudE的相互作用调控神经元迁移。LIS1抗体被用于检测小鼠脑组织中的LIS1表达分布，证实其在皮层分层中的关键作用。
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2. **文献名称**: *"Disruption of neural progenitors along the ventricular zone leads to dysplasia in the cerebral cortex"*
**作者**: Moon H.M., Wynshaw-Boris A.
**摘要**: 利用LIS1抗体进行免疫荧光染色，研究显示LIS1缺失会导致神经前体细胞排列紊乱和皮层发育畸形，提示LIS1在维持放射状胶质细胞极性中的功能。
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3. **文献名称**: *"LIS1 and DCX: Implications for brain development and human disease in relation to microtubules"*
**作者**: Tanaka T. et al.
**摘要**: 通过体外微管结合实验和免疫组织化学，该文献证明LIS1抗体标记的蛋白与微管动力学的调控相关，并揭示了LIS1/Dynein复合物在神经元迁移中的协同机制。
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4. **文献名称**: *"LIS1 RNA interference blocks neural stem cell division and causes cortical dysplasia in zebrafish"*
**作者**: Tsai J.W. et al.
**摘要**: 使用LIS1抗体验证斑马鱼模型中LIS1敲低的效果，发现其导致神经干细胞分裂异常及皮层结构缺陷，支持LIS1在进化保守的神经发育通路中的作用。
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以上文献均以LIS1抗体为核心工具，研究其在神经元迁移、皮层发育及疾病机制中的功能。
The LIS1 antibody targets the LIS1 protein, encoded by the *PAFAH1B1* gene, which plays a critical role in neuronal migration and brain development. LIS1 was first identified in 1993 as the gene disrupted in Miller-Dieker syndrome (MDS), a severe neurodevelopmental disorder characterized by lissencephaly ("smooth brain") due to defective cortical neuron migration. The protein consists of two main domains: an N-terminal β-propeller involved in protein interactions and a C-terminal α-helical domain. LIS1 interacts with components of the cytoplasmic dynein complex, regulating microtubule dynamics and intracellular transport, essential for proper neuronal positioning during embryogenesis.
Antibodies against LIS1 are widely used in research to study neurodevelopmental mechanisms, assess protein expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence, and validate genetic models of lissencephaly. Dysregulation of LIS1 is linked not only to MDS but also to epilepsy, autism spectrum disorders, and other cortical malformations. Additionally, LIS1 interacts with proteins such as DCX and NDEL1. forming pathways critical for cytoskeletal organization. Clinically, LIS1 antibodies aid in diagnosing lissencephaly-related mutations and understanding genotype-phenotype correlations. Their application extends to exploring LIS1’s role beyond the brain, including roles in cell division and cancer biology.
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