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Rabbit Monoclonal RHEB Antibody

  • 中文名： RHEB抗体
  • 别    名： RHEB 2; Rheb; RHEB2;;RHEB
货号: IPDX18287
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/1000-1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRHEB 2; Rheb; RHEB2;;RHEB
WB Predicted band size20 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human RHEB
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于RHEB抗体的3篇代表性文献，简要概括其内容：

1. **文献名称**: "Rheb promotes cell growth as a component of the insulin/TOR signalling network"

**作者**: Saucedo, L.J. 等 (2003)

**摘要**: 该研究揭示了RHEB在胰岛素/TOR信号通路中促进细胞生长的作用，通过果蝇模型和哺乳动物细胞实验，利用特异性RHEB抗体验证了其在调控细胞周期和增殖中的关键功能。

2. **文献名称**: "Rheb is essential for murine development"

**作者**: Goorden, S.M. 等 (2011)

**摘要**: 研究通过基因敲除小鼠模型，结合RHEB抗体的免疫组织化学分析，证实RHEB对胚胎发育至关重要，并发现其缺失导致神经元发育异常和胚胎致死。

3. **文献名称**: "Targeting RHEB in cancer: Current insights and therapeutic opportunities"

**作者**: Deng, L. 等 (2020)

**摘要**: 综述了RHEB在肿瘤发生中的作用，总结了利用RHEB抗体检测其在多种癌症中的表达水平的研究，并探讨靶向RHEB-mTORC1通路的治疗策略。

这些文献均涉及RHEB抗体的应用，涵盖发育生物学、癌症研究及分子机制探索。

背景信息

The RHEB (Ras homolog enriched in brain) antibody is a crucial tool for studying the RHEB protein, a small GTPase belonging to the Ras superfamily. RHEB plays a pivotal role in regulating the mTORC1 (mechanistic target of rapamycin complex 1) signaling pathway, which controls cell growth, proliferation, autophagy, and metabolism in response to nutrients and growth signals. Dysregulation of RHEB-mTORC1 signaling is implicated in cancers, neurological disorders, and metabolic diseases. Researchers use RHEB antibodies to detect endogenous RHEB expression levels, subcellular localization (primarily in lysosomal and endosomal membranes), and post-translational modifications (e.g., farnesylation required for membrane association). These antibodies are widely applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specific RHEB antibodies may distinguish between inactive GDP-bound and active GTP-bound states, though many detect total RHEB regardless of activation status. Cross-reactivity varies; some antibodies recognize human, mouse, or rat RHEB isoforms. Notably, phosphorylation of RHEB at specific residues (e.g., Ser113) under certain conditions may affect antibody binding. Validation using knockout cell lines or siRNA-mediated knockdown is critical to confirm specificity, as non-specific bands are common in Western blots. Key studies using RHEB antibodies have elucidated its role in tuberous sclerosis complex (TSC) pathology and rapamycin-sensitive signaling cascades.

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