首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人角蛋白II型细胞骨架6A(KRT6A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KRT6A含量。KRT6A是一个靶点，在生物体内它属于角蛋白相关基因，角蛋白在维持细胞结构和功能方面发挥重要作用。目前研究可能聚焦于其在细胞增殖、分化等生理过程中的机制，以及其在疾病如某些皮肤病发生发展中的作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础医学研究中KRT6A蛋白在皮肤疾病模型、肿瘤微环境分析、细胞应激反应等场景的动态监测，可配合基因编辑、药物干预等实验方案开展分子机制探索，为上皮细胞生物学研究提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CK-6A ELISA Kit; CK-6D ELISA Kit; CK6A ELISA Kit; CK6C ELISA Kit; CK6D ELISA Kit; Cytokeratin-6A ELISA Kit; Cytokeratin-6D ELISA Kit; K2C6A_HUMAN ELISA Kit; K6A ELISA Kit; K6C ELISA Kit; K6D ELISA Kit; keratin 6A ELISA Kit; Keratin ELISA Kit; Keratin; type II cytoskeletal 6A ELISA Kit; Keratin-6A ELISA Kit; Krt6a ELISA Kit; KRT6C ELISA Kit; KRT6D ELISA Kit; type II cytoskeletal 6A ELISA Kit; Type-II keratin Kb6 ELISA Kit
缩写：KRT6A
Uniprot No.：P02538
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KRT6A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 98-108 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of human KRT6A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-103 | |||||
| EDTA plasma (n=4) | 94 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.0982.1022.1001.95551.7211.7931.7571.6122.51.3821.4211.4021.2571.251.0901.1501.1200.9750.6250.6950.7140.7050.5600.3120.4590.4360.4480.3030.1560.2670.2620.2650.12000.1420.1470.145 | |||||||
货期：3-5 working days
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