首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血浆激肽释放酶(KLKB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的KLKB1含量。KLKB1，即血浆激肽释放酶（KLKB1），是一种参与血液凝固、纤维蛋白溶解、激肽生成和炎症的表面依赖激活的糖蛋白。其研究机制涉及基因突变、多态性与疾病如肺血栓栓塞症的相关性，以及通过CRISPR-Cas9基因编辑技术治疗遗传性血管性水肿等。试剂盒检测范围为9.38 mU/mL-600 mU/mL，该产品特别适用于基础科研领域，可用于研究KLKB1在心血管疾病、炎症反应、纤维化疾病中的功能调控机制，或用于评估药物干预对激肽释放酶系统的影响，为探索相关疾病的分子机制及潜在治疗靶点提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fletcher factor ELISA Kit; kallikrein B plasma ELISA Kit; Kallikrein B plasma (Fletcher factor) 1 ELISA Kit; Kallikrein B; plasma 1 ELISA Kit; Kallikrein B1 ELISA Kit; Kininogenin ELISA Kit; KLK3 ELISA Kit; KLKB1 ELISA Kit; KLKB1_HUMAN ELISA Kit; PKK ELISA Kit; PKKD ELISA Kit; Plasma kallikrein ELISA Kit; Plasma kallikrein B1 ELISA Kit; Plasma kallikrein light chain ELISA Kit; Plasma prekallikrein ELISA Kit; PPK ELISA Kit; Prekallikrein ELISA Kit
缩写：KLKB1
Uniprot No.：P03952
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：9.38 mU/mL-600 mU/mL
灵敏度：2.34 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 89-100 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 83-93 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 80-103 | ||||
| EDTA plasma (n=4) | 96 | 86-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected6002.1012.2072.1542.0483001.6951.6871.6911.5851501.2611.2781.2701.164750.8420.8670.8550.74937.50.5030.5140.5090.40318.750.3450.3680.3570.2519.380.2010.2110.2060.10000.1050.1070.106 | ||||||
货期：3-5 working days
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