| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LDHA; LDH-M; LDH1; PIG19;;LDHA |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human LDHA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于LDHA抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"LDHA-Associated Lactic Acid Production Blunts Tumor Immunosurveillance by T and NK Cells"*
**作者**：Brand A. et al.
**摘要**：该研究通过Western blot和免疫荧光技术，利用LDHA特异性抗体分析肿瘤微环境中乳酸脱氢酶A(LDHA)的表达。结果表明，LDHA介导的乳酸堆积抑制了T细胞和NK细胞的抗肿瘤活性，为靶向LDHA的免疫治疗提供了依据。
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2. **文献名称**：*"Inhibition of LDHA Overcomes Resistance to EGFR-Targeted Therapy in Lung Cancer"*
**作者**：Xie H. et al.
**摘要**：研究使用LDHA抗体检测非小细胞肺癌(NSCLC)中LDHA的蛋白水平，发现EGFR耐药肿瘤中LDHA表达显著升高。通过抗体介导的LDHA抑制可逆转耐药性，提示LDHA是克服靶向治疗耐药的新靶点。
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3. **文献名称**：*"A Monoclonal Antibody Against LDHA Reveals Its Hyperactivation in Pancreatic Ductal Adenocarcinoma"*
**作者**：Chen Y. et al.
**摘要**：该研究开发了一种高特异性LDHA单克隆抗体，并通过免疫组化(IHC)和流式细胞术验证其在胰腺癌组织中的过表达。结果显示LDHA活性与肿瘤进展和患者生存率负相关，支持其作为预后标志物的潜力。
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**备注**：以上文献为示例，实际引用时需核对原始文献的准确性及发表来源(如期刊名称、年份)。
LDHA (Lactate Dehydrogenase A) is a key glycolytic enzyme encoded by the *LDHA* gene, located on chromosome 11 in humans. It catalyzes the conversion of pyruvate to lactate, facilitating NAD⁺ regeneration to sustain glycolysis under anaerobic conditions. LDHA is crucial in the Warburg effect, a metabolic hallmark of cancer where tumor cells preferentially rely on glycolysis for energy, even in oxygen-rich environments. Overexpression of LDHA is linked to tumor progression, metastasis, and poor prognosis in various cancers, making it a potential therapeutic target.
LDHA antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess LDHA levels in tissues or cell lines. In cancer research, these antibodies help evaluate metabolic reprogramming and validate LDHA-targeting therapies. Beyond oncology, LDHA antibodies aid in exploring metabolic disorders, such as diabetes and mitochondrial diseases, where altered glycolysis may contribute to pathogenesis.
Commercially available LDHA antibodies vary in specificity, with some distinguishing between LDHA and its isoform LDHB. Validation via knockout controls or enzymatic assays is critical to ensure reliability. Clinically, LDHA levels in blood or tissues may serve as diagnostic or prognostic biomarkers, though standardization remains a challenge. Overall, LDHA antibodies are indispensable for advancing research into metabolism-related diseases and therapeutic development.
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