首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人激肽释放酶7(KLK 7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KLK7含量。KLK7是一个重要靶点。它属于激肽释放酶家族成员，在皮肤角质形成、炎症反应等生理病理过程中发挥作用。研究其机制主要聚焦于其活性调节、与其他信号分子相互作用等，有望为皮肤相关疾病如特应性皮炎等的治疗带来新思路。试剂盒检测范围为0.625 ng/mL-40 ng/mL，产品适用于探索KLK7在皮肤屏障功能异常、肿瘤微环境重塑或慢性炎症疾病中的表达调控机制，为细胞模型、动物实验或人体组织样本的蛋白水平研究提供技术支持，助力于疾病相关分子通路的机制解析及生物标志物筛选等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Chymotryptic stratum corneum ELISA Kit; hK 7 ELISA Kit; hK7 ELISA Kit; hSCCE ELISA Kit; Kallikrein 7 (chymotryptic stratum corneum) ELISA Kit; Kallikrein related peptidase 7 ELISA Kit; Kallikrein-7 ELISA Kit; Kallikrein7 ELISA Kit; KLK 7 ELISA Kit; Klk7 ELISA Kit; KLK7_HUMAN ELISA Kit; Protease serine 6 ELISA Kit; PRSS 6 ELISA Kit; PRSS6 ELISA Kit; SCCE ELISA Kit; Serine protease 6 ELISA Kit; Signal protein ELISA Kit; Stratum corneum chymotryptic enzyme ELISA Kit
缩写：KLK7
Uniprot No.：P49862
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||
| Sample | Serum(n=4) | ||||
| 1:1 | Average % | 102 | |||
| Range % | 92-108 | ||||
| 1:2 | Average % | 94 | |||
| Range % | 91-97 | ||||
| 1:4 | Average % | 92 | |||
| Range % | 86-98 | ||||
| 1:8 | Average % | 97 | |||
| Range % | 92-103 | ||||
回收率：
| The recovery of human KLK 7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||
| Sample Type | Average % Recovery | Range | |||
| Serum (n=5) | 104 | 98-108 | |||
| EDTA plasma (n=4) | 94 | 90-98 | |||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||
| ng/mlOD1OD2AverageCorrected402.7082.6072.6582.514201.8651.7641.8151.671101.2171.2061.2121.06850.8110.8400.8260.6822.50.4580.4470.4530.3091.250.3220.3110.3170.1730.6250.2350.2320.2340.09000.1440.1430.144 | |||||
货期：3-5 working days
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