| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EMI1; FBX5; Fbxo31; fbxo5;;FBXO5 |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FBXO5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Emi1抗体的3篇代表性文献(简要概括)：
1. **文献名称**：*The Emi1 protein inhibits the anaphase-promoting complex/cyclosome by blocking the binding of the E2C UbcH10*
**作者**：Hsu JY, et al.
**摘要内容**：研究通过免疫沉淀(Emi1抗体)和Western blot技术，揭示了Emi1通过竞争性结合APC/C复合物的E2连接酶UbcH10.调控细胞周期进程的分子机制。
2. **文献名称**：*Control of Emi1 activity in the oocyte through phosphorylation at the meiotic divisions*
**作者**：Reis A, et al.
**摘要内容**：利用Emi1特异性抗体进行免疫荧光和蛋白质印迹，发现Emi1的磷酸化状态在减数分裂过程中调控其稳定性及APC/C抑制功能，影响卵母细胞成熟。
3. **文献名称**：*Overexpression of Emi1 promotes genomic instability and tumorigenesis*
**作者**：Lehman NL, et al.
**摘要内容**：通过Emi1抗体检测发现，Emi1在多种癌细胞中异常高表达，其过表达导致染色体错误分离和基因组不稳定性，提示其在肿瘤发生中的作用。
**备注**：以上文献均以Emi1抗体为工具，研究其在细胞周期、癌症或生殖生物学中的功能。如需具体实验细节(如抗体来源、实验条件)，建议进一步查阅原文方法部分。
**Background of Emi1 Antibody**
Emi1 (Early Mitotic Inhibitor 1), also known as FBXO5. is a critical regulator of the cell cycle, primarily functioning as an inhibitor of the anaphase-promoting complex/cyclosome (APC/C). Discovered for its role in delaying APC/C activation until metaphase, Emi1 ensures proper progression through mitosis by preventing premature degradation of cyclin B and securin, proteins essential for sister chromatid separation. Structurally, Emi1 contains an F-box domain and a conserved inhibitory region (C-terminal loop) responsible for APC/C binding and suppression.
Dysregulation of Emi1 is linked to genomic instability and oncogenesis. Overexpression of Emi1 disrupts mitotic timing, leading to tetraploidy and chromosomal abnormalities, commonly observed in cancers such as breast, ovarian, and prostate tumors. Conversely, Emi1 depletion triggers unscheduled APC/C activation, resulting in mitotic collapse.
Emi1 antibodies are essential tools for studying cell cycle dynamics, protein interactions, and cancer mechanisms. They enable detection of Emi1 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers also utilize these antibodies to explore therapeutic strategies targeting Emi1-overexpressing cancers. Given its dual role as a checkpoint regulator and oncogenic driver, Emi1 remains a focal point in cell biology and oncology research.
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