首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人激肽释放酶2(hK 2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KLK2含量。KLK2即激肽释放酶2，是前列腺特异的丝氨酸蛋白酶。它在前列腺组织中高度表达，与前列腺癌的发生发展密切相关。研究机制上，它可参与细胞外基质降解、细胞增殖与凋亡调控等过程，有望成为前列腺癌诊断与治疗的潜在靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，科研领域可将其应用于前列腺癌相关分子机制研究、疾病标志物筛选、药物疗效评估或病理模型分析等方向，例如通过检测患者体液或组织样本中hK 2的动态变化，探索其与肿瘤侵袭性、转移潜能等生物学行为的关联性，为癌症诊疗的科研探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Glandular kallikrein 2 ELISA Kit; Glandular kallikrein-1 ELISA Kit; hGK 1 ELISA Kit; hGK-1 ELISA Kit; hK2 ELISA Kit; Kallikrein 2 prostatic ELISA Kit; Kallikrein related peptidase 2 ELISA Kit; kallikrein, glandular ELISA Kit; Kallikrein, prostatic ELISA Kit; Kallikrein-2 ELISA Kit; KLK 2 ELISA Kit; Klk-2 ELISA Kit; Klk2 ELISA Kit; KLK2_HUMAN ELISA Kit; KLK2A 2 ELISA Kit; KLK2A2 ELISA Kit; MGC12201 ELISA Kit; Tissue kallikrein 2 ELISA Kit; Tissue kallikrein-2 ELISA Kit; Ton ELISA Kit
缩写：KLK2
Uniprot No.：P20151
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human hK 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 94-107 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of human hK 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.3712.2012.2862.18020001.9731.9211.9471.84110001.3121.4051.3591.2535000.7120.7320.7220.6162500.3370.3420.3400.2341250.2560.2610.2590.15362.50.1970.1990.1980.09200.1050.1070.106 | |||||||
货期：3-5 working days
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