| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MCAK; KNSL6;;KIF2C |
| WB Predicted band size | 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human KIF2C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于MCAK抗体的参考文献及其摘要概括：
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1. **文献名称**: *"MCAK is a potent regulator of mitotic microtubule dynamics"*
**作者**: Wordeman L., Mitchison T.J.
**摘要**: 该研究利用MCAK抗体进行免疫荧光和Western blot分析，发现MCAK蛋白通过其微管解聚活性调控有丝分裂中纺锤体微管的动态平衡，对染色体排列和分离至关重要。
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2. **文献名称**: *"KIF2C/MCAK promotes chromosome congression and cancer cell proliferation"*
**作者**: Zhang X., et al.
**摘要**: 通过MCAK抗体抑制实验，研究发现MCAK在多种癌细胞中高表达，并通过调节微管稳定性促进染色体定向移动和快速增殖，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**: *"Interplay between Aurora A kinase and MCAK in controlling spindle assembly"*
**作者**: Jang C.Y., et al.
**摘要**: 该研究结合MCAK抗体与活细胞成像技术，揭示了Aurora A激酶通过磷酸化修饰MCAK的活性，进而影响纺锤体组装和细胞周期进程的分子机制。
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这些文献覆盖了MCAK在微管动力学、癌症生物学及激酶调控网络中的功能研究，均通过MCAK抗体实现了蛋白定位、表达及功能分析。如需具体期刊信息或发表年份，可进一步补充检索。
**Background of MCAK Antibody**
MCAK (Mitotic Centromere-Associated Kinesin), also known as KIF2C, is a member of the kinesin-13 family of microtubule-depolymerizing motor proteins. It plays a critical role in regulating microtubule dynamics during mitosis, ensuring proper chromosome alignment, segregation, and faithful cell division. MCAK localizes to centromeres, spindle poles, and microtubule tips, where it destabilizes microtubules by catalyzing the removal of tubulin subunits, thus correcting erroneous kinetochore-microtubule attachments and maintaining genomic stability.
Antibodies targeting MCAK are essential tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate MCAK's role in mitotic regulation, cancer biology, and chromosomal instability. Dysregulation of MCAK has been linked to tumorigenesis, metastasis, and chemoresistance, making it a potential biomarker and therapeutic target in oncology.
MCAK antibodies also aid in exploring post-translational modifications (e.g., phosphorylation by Aurora kinases) that modulate its activity. Their specificity and reliability enable researchers to dissect molecular mechanisms underlying mitotic errors and diseases, advancing both basic research and translational applications in cell biology and cancer therapeutics.
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