首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子Ⅱ型肺泡细胞表面抗原(KL-6)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KL-6含量。KL-6，也称为涎液化糖链抗原，是一种表达于II型肺泡上皮细胞的糖蛋白，被认为是诊断间质性肺疾病（ILD）的准确性最高的生物标记物。其表达增多与肺泡上皮细胞增生和肺泡基底膜损伤有关，在ILD的早期诊断和病情评估中具有重要价值。试剂盒检测范围为50 IU/mL-800 IU/mL，支持科研人员在体外实验中定量评估肺损伤模型、药物干预研究或疾病生物标志物探索中的 KL - 6 动态变化，为呼吸系统疾病的基础研究提供灵敏可靠的工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：KL-6
Uniprot No.：
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：50 IU/mL-800 IU/mL
灵敏度：25 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit KL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rabbit KL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 89 | 87-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2Average8000.1910.1980.1954000.3510.3370.3442000.6140.6270.6211001.1541.1321.143502.3302.2242.277 | ||||||
货期：3-5 working days
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