| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DDX2; DDX2A; eIF 4A I; EIF4A; eIF4A I; eIF4A-I; EIF4A1;;eIF4A1 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human eIF4A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于eIF4A1抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*eIF4A1 is a critical mediator of metabolic reprogramming and chemoresistance in triple-negative breast cancer*
**作者**：Chen, H.H., et al.
**摘要**：该研究利用eIF4A1特异性抗体，通过Western blot和免疫组化技术，发现eIF4A1在三阴性乳腺癌中显著高表达，且与化疗耐药性相关。抑制eIF4A1可逆转肿瘤细胞的代谢异常并增强化疗敏感性。
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2. **文献名称**：*Targeting eIF4A1 with rocaglates inhibits melanoma progression through translational repression of oncogenic proteins*
**作者**：Rubio, C.A., et al.
**摘要**：研究通过eIF4A1抗体验证其在黑色素瘤中的功能，发现小分子抑制剂(如RocA)通过结合eIF4A1抑制其RNA解旋酶活性，从而选择性阻断促癌蛋白的翻译，抑制肿瘤生长和转移。
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3. **文献名称**：*Immunohistochemical analysis of eIF4A1 in hepatocellular carcinoma: correlation with clinical outcomes*
**作者**：Wang, L., et al.
**摘要**：该研究利用eIF4A1抗体对肝细胞癌组织进行免疫组化分析，发现eIF4A1的高表达与患者总生存期缩短和肿瘤复发风险升高显著相关，提示其可作为预后生物标志物。
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以上文献均通过eIF4A1抗体在实验中对蛋白表达或功能进行解析，涵盖癌症机制、治疗靶点及临床预后等领域。
The eukaryotic initiation factor 4A1 (eIF4A1) is a critical ATP-dependent RNA helicase and a core component of the eIF4F complex, which regulates the cap-dependent translation initiation in eukaryotes. As a member of the DEAD-box protein family, eIF4A1 facilitates ribosome recruitment to mRNA by unwinding secondary structures in the 5' untranslated region (UTR) of transcripts. Its activity is tightly coordinated with other translation initiation factors, such as eIF4E and eIF4G, and modulated by signaling pathways like mTOR. Dysregulation of eIF4A1 has been implicated in cancer, neurodegeneration, and viral infection due to its role in controlling the translation of oncoproteins, stress-response proteins, or viral RNAs.
Antibodies targeting eIF4A1 are widely used to study translational regulation, protein synthesis dynamics, and cellular stress responses. They enable the detection of eIF4A1 expression levels, subcellular localization, and interactions within the eIF4F complex through techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific eIF4A1 antibodies are also employed to validate RNA helicase inhibition by compounds such as silvestrol or hippuristanol in cancer research. Notably, eIF4A1-specific antibodies must distinguish it from its paralog eIF4A2. which shares ~90% sequence homology but exhibits distinct functional roles. These tools have advanced studies on translation-driven pathologies and therapeutic targeting of the eIF4F complex in diseases characterized by aberrant protein synthesis.
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