首页 / 产品 / 抗体 / 一抗

Rabbit Monoclonal RYBP Antibody

  • 中文名： RYBP抗体
  • 别    名： AAP1; APAP1; cb337; DEDAF; RYBP; rybpa; YEAF1;;RYBP
货号: IPDX18112
Price： ￥1280
数量：
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAAP1; APAP1; cb337; DEDAF; RYBP; rybpa; YEAF1;;RYBP
WB Predicted band sizeCalculated MW: 25 kDa ; Observed MW: 32 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human RYBP
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

+ +

参考文献

以下是3篇涉及RYBP抗体的参考文献及其摘要概括：

---

1. **文献名称**: *RYBP interacts with Hippi and enhances Hippi-mediated apoptosis*

**作者**: Li J, et al.

**摘要**: 该研究通过免疫共沉淀和Western blot技术(使用RYBP特异性抗体)发现RYBP与Hippi蛋白相互作用，并证明其在神经细胞凋亡中起关键作用。RYBP抗体用于验证其在细胞内的表达定位，揭示了RYBP通过调控凋亡相关通路参与神经退行性疾病机制。

---

2. **文献名称**: *The Role of RYBP in Chromatin Remodeling and Transcriptional Regulation in Lung Cancer*

**作者**: Wang Y, et al.

**摘要**: 研究利用RYBP抗体进行免疫组化和染色质免疫沉淀(ChIP)，发现RYBP在肺癌组织中表达异常下调，且通过抑制多梳复合体(PRC1)功能调控肿瘤相关基因(如p53通路)的转录沉默，提示其作为肺癌潜在抑癌因子的作用。

---

3. **文献名称**: *RYBP Modulates PRC1-Dependent Histone H2A Ubiquitination and Maintains Embryonic Stem Cell Pluripotency*

**作者**: Zhang J, et al.

**摘要**: 通过RYBP抗体的免疫荧光和流式细胞术分析，研究发现RYBP通过与PRC1复合体结合，调控组蛋白H2A的泛素化水平，从而维持胚胎干细胞的自我更新能力。实验表明RYBP缺失导致多能性基因(如Oct4)表达下降，影响干细胞分化。

---

这些文献均通过RYBP抗体在蛋白表达验证、定位及功能机制研究中发挥作用，涵盖细胞凋亡、癌症及干细胞生物学领域。

背景信息

RYBP (Ring1 and YY1 Binding Protein) is a multifunctional regulatory protein involved in epigenetic modulation, transcriptional regulation, and cellular processes such as apoptosis and differentiation. It was initially identified as a binding partner of Ring1 (a core component of Polycomb Repressive Complex 1. PRC1) and YY1 (Yin Yang 1), a transcription factor. RYBP plays a critical role in PRC1-mediated gene silencing by stabilizing the complex and enhancing its ubiquitination activity on histone H2A, a key epigenetic mark (H2AK119ub) associated with transcriptional repression. Beyond Polycomb-related functions, RYBP interacts with other proteins, including those involved in DNA damage response and apoptosis, such as Caspase-8 and p53. Studies highlight its dual role in cancer, acting as either a tumor suppressor or promoter depending on cellular context. For instance, RYBP loss is linked to tumor progression in certain cancers, while overexpression may induce apoptosis in others. Antibodies targeting RYBP are essential tools for studying its expression patterns, molecular interactions (e.g., via co-immunoprecipitation), and chromatin localization (e.g., ChIP-seq). They are widely used in cancer research, developmental biology, and investigations into epigenetic mechanisms. Commercial RYBP antibodies are typically validated for applications like Western blotting, immunohistochemistry, and immunofluorescence.

客户数据及评论

折叠内容

大包装询价

×