| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDCA1; CT106; hNuf2; hNuf2R; hsNuf2; NUF2; NUF2R;;NUF2 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NUF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是假设的关于Nuf2抗体的参考文献示例(非真实文献，仅作格式参考)：
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1. **《Nuf2 Antibody Validation in Mitotic Chromosome Segregation Studies》**
*作者：Smith A, et al. (2020)*
摘要：本研究验证了一种特异性Nuf2抗体的有效性，通过免疫印迹和免疫荧光技术证实其在HeLa细胞有丝分裂过程中能准确标记动粒蛋白Nuf2.为研究染色体分离机制提供工具支持。
2. **《Elevated Nuf2 Expression Correlates with Poor Prognosis in Hepatocellular Carcinoma》**
*作者：Li X, et al. (2018)*
摘要：利用Nuf2抗体对肝癌组织进行免疫组化分析，发现Nuf2蛋白在肿瘤组织中高表达，且与患者生存率显著负相关，提示其可作为肝癌潜在预后标志物。
3. **《Functional Analysis of Nuf2 in Kinetochore-Microtubule Attachment Using RNAi and Antibody-Based Knockdown》**
*作者：Tanaka K, et al. (2015)*
摘要：结合RNA干扰和Nuf2抗体显微注射技术，证明Nuf2缺失导致动粒-微管附着异常，引发染色体排列错误，阐明其在细胞分裂中的关键作用。
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注：以上文献为示例，实际引用时需通过PubMed/Web of Science等平台检索真实文献，建议使用关键词“Nuf2 antibody”“kinetochore”“cancer prognosis”等筛选近年研究。
Nuf2 (Nuclear division cycle 2 homolog) is a critical component of the Ndc80 complex, a conserved kinetochore-associated protein complex essential for proper chromosome segregation during mitosis and meiosis. The Ndc80 complex, composed of Nuf2. Ndc80/Hec1. Spc24. and Spc25. mediates the attachment of spindle microtubules to kinetochores, ensuring accurate chromosome alignment and segregation. Dysregulation of Nuf2 or its associated partners can lead to chromosomal instability, a hallmark of many cancers and genetic disorders.
Nuf2 antibodies are immunological tools widely used to study the expression, localization, and function of the Nuf2 protein in cellular processes. These antibodies are particularly valuable in cancer research, as elevated Nuf2 expression has been linked to tumor progression, poor prognosis, and therapeutic resistance in various cancers, including breast, liver, and colorectal cancers. By employing techniques like Western blotting, immunofluorescence, or immunohistochemistry, researchers utilize Nuf2 antibodies to investigate its role in cell cycle regulation, kinetochore-microtubule dynamics, and mitotic checkpoint signaling. Additionally, they aid in exploring Nuf2's potential as a diagnostic biomarker or therapeutic target. Validation of Nuf2 antibody specificity is crucial, often achieved through knockout/knockdown controls or epitope mapping, to ensure accurate interpretation of experimental results. Recent studies also focus on Nuf2's interaction with checkpoint proteins and its responsiveness to anti-mitotic drugs.
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