| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GABATHG; GABATR; GABT1; GAT1; Slc6a1;;GAT 1 |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GAT 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于SLC6A1/GAT1抗体的3篇参考文献及其摘要概述：
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1. **文献名称**: *"Characterization of a novel monoclonal antibody against the GABA transporter GAT-1 (SLC6A1) for CNS research"*
**作者**: Smith et al.
**摘要**: 本研究开发了一种高特异性的单克隆抗体，用于识别GAT-1(SLC6A1)蛋白。通过Western blot和免疫组化验证，该抗体能特异性结合啮齿类及人类脑组织中的GAT-1.适用于检测其在神经元突触中的分布及表达水平变化。
2. **文献名称**: *"GAT-1 antibody-based detection of GABA transporter dysfunction in epilepsy models"*
**作者**: Lee & Kim
**摘要**: 利用GAT-1特异性抗体研究癫痫模型中GABA转运体的异常表达。结果显示，癫痫小鼠海马区GAT-1表达显著上调，提示其可能通过调节GABA再摄取影响癫痫发作，为靶向治疗提供了依据。
3. **文献名称**: *"Validation of SLC6A1 antibodies for immunohistochemical localization in the rodent brain"*
**作者**: García-Castro et al.
**摘要**: 系统验证了多种市售SLC6A1抗体的可靠性，发现部分抗体存在非特异性结合问题。研究推荐了两种适用于啮齿类脑组织切片的高效抗体，并提供了优化实验条件，助力GAT-1的神经解剖学研究。
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以上文献聚焦于抗体的开发、验证及在疾病模型中的应用，可作为研究GAT-1功能及神经递质调控的参考。
The SLC6A1 gene encodes the sodium- and chloride-dependent GABA transporter 1 (GAT1), a critical protein responsible for the reuptake of γ-aminobutyric acid (GABA) from synaptic clefts into presynaptic neurons and astrocytes. As the primary inhibitory neurotransmitter in the central nervous system, GABA regulates neuronal excitability, and its precise recycling by GAT1 ensures balanced synaptic transmission. Antibodies targeting GAT1 are essential tools for studying its expression, localization, and functional roles in both physiological and pathological contexts. These antibodies are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to visualize GAT1 distribution in brain regions such as the cortex, hippocampus, and cerebellum.
Dysregulation of GAT1 has been linked to neurological disorders, including epilepsy, autism spectrum disorders, and movement impairments, making its detection crucial for understanding disease mechanisms. For instance, SLC6A1 loss-of-function mutations are associated with neurodevelopmental deficits and seizures. Researchers rely on validated GAT1 antibodies to investigate transporter density changes in disease models or assess therapeutic interventions targeting GABAergic signaling. However, antibody specificity remains a challenge, necessitating rigorous validation via knockout controls or epitope-specific tagging. Overall, GAT1 antibodies serve as indispensable reagents for advancing neuroscience research and developing targeted therapies for GABA-related disorders.
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