| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VPS24; CGI149; CHMP3; hVps24; NEDF;;VPS24 |
| WB Predicted band size | Calculated MW: 25 kDa ; Observed MW: 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VPS24 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及VPS24抗体的研究文献摘要(内容基于公开研究整合，非真实文献引用，仅供参考)：
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1. **标题**: *ESCRT-III protein VPS24 regulates endosomal sorting through polymer-dependent membrane remodeling*
**作者**: Smith J, et al.
**摘要**: 研究使用VPS24抗体进行Western blot和免疫荧光，发现VPS24通过形成动态聚合物参与内体膜重塑，调控病毒颗粒出芽及多泡体生成。
2. **标题**: *Mechanistic insights into VPS24 function in ESCRT-III complex assembly*
**作者**: Lee S, Chen R.
**摘要**: 通过VPS24特异性抗体阻断实验，揭示了VPS24与其他ESCRT-III亚基(如CHMP3)的互作机制，证明其在细胞分裂中的膜分离作用。
3. **标题**: *VPS24 knockdown impairs autophagy via disrupting lysosomal biogenesis*
**作者**: Wang Y, et al.
**摘要**: 利用VPS24抗体进行免疫共沉淀，发现VPS24缺失导致溶酶体成熟障碍，影响自噬小体降解过程，表明其与自噬调控相关。
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**注**：以上文献信息为示例性质，实际研究中建议通过PubMed或Google Scholar以关键词“VPS24 antibody”、“ESCRT-III function”检索近期论文。
The VPS24 antibody is a research tool used to detect VPS24 (Vacuolar Protein Sorting 24), a component of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery, specifically the ESCRT-III subcomplex. VPS24. also known as CHMP3 (Charged Multivesicular Body Protein 3), plays a critical role in membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, and viral budding. It facilitates the sorting of ubiquitinated cargo proteins into intraluminal vesicles of MVBs, which is essential for lysosomal degradation, receptor downregulation, and exosome biogenesis.
Antibodies targeting VPS24 are widely employed in studies investigating ESCRT-III dynamics, membrane scission mechanisms, and diseases linked to ESCRT dysfunction, such as cancer, neurodegenerative disorders, and viral infections (e.g., HIV-1 budding). These antibodies are typically validated in applications like Western blotting, immunofluorescence, and immunoprecipitation, often using cell lines or tissues with modulated ESCRT-III expression. Researchers also utilize VPS24 antibodies to explore its interaction with other ESCRT components (e.g., CHMP2. VPS4 ATPase) and its role in cellular stress responses or apoptosis. Commercial VPS24 antibodies are often raised against recombinant protein fragments or synthetic peptides, with specificity confirmed via knockout validation. Its study contributes to understanding membrane trafficking pathologies and therapeutic targeting of ESCRT-related pathways.
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