首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述:人整合素αM型(ITG αM/CD11b)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ITGAM含量。ITGAM是重要靶点,它编码整合素αM亚基,在免疫系统中发挥关键作用。研究发现其参与白细胞粘附、迁移等过程,与多种炎症、免疫性疾病相关。深入研究其作用机制,有助于为相关疾病开发更有效的治疗策略。试剂盒检测范围为0.78 ng/mL-50 ng/mL,适用于免疫学基础研究,如巨噬细胞功能分析、炎症机制探索或药物研发中免疫调节效果的体外评估,也可用于动物模型中组织局部免疫反应的定量分析。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:antigen CD11b (p170) ELISA Kit; Antigen CD11b p170 ELISA Kit; CD11 antigen like family member B ELISA Kit; CD11 antigen-like family member B ELISA Kit; CD11b ELISA Kit; CD11b/CD18 ELISA Kit; CD49d ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Complement component 3 receptor 3 subunit ELISA Kit; Complement Component Receptor 3 Alpha ELISA Kit; Complement receptor type 3 ELISA Kit; Complement receptor type 3, alpha subunit ELISA Kit; CR 3 alpha chain (CR3A) ELISA Kit; CR 3 alpha chain ELISA Kit; CR-3 alpha chain ELISA Kit; CR3 ELISA Kit; CR3A ELISA Kit; F730045J24Rik ELISA Kit; Integrin Alpha M ELISA Kit; Integrin alpha M chain ELISA Kit; Integrin alpha-M ELISA Kit; Integrin beta 2 alpha subunit ELISA Kit; Integrin subunit alpha M ELISA Kit; integrin, alpha M (complement component 3 receptor 3 subunit) ELISA Kit; ITAM_HUMAN ELISA Kit; ITGAM ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; Ly-40 ELISA Kit; MAC 1 ELISA Kit; Mac-1a ELISA Kit; MAC1 ELISA Kit; Mac1, alpha subunit ELISA Kit; MAC1A ELISA Kit; Macrophage antigen alpha polypeptide ELISA Kit; MGC117044 ELISA Kit; Mo1 ELISA Kit; Mo1, alpha subunit ELISA Kit; MO1A ELISA Kit; Neutrophil adherence receptor alpha M subunit ELISA Kit; Neutrophil adherence receptor ELISA Kit; SLEB6 ELISA Kit
缩写:ITGAM
Uniprot No.:P11215
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.78 ng/mL-50 ng/mL
灵敏度:0.195 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ITG/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 100 | ||||
Range % | 95-104 | |||||
1:2 | Average % | 98 | ||||
Range % | 94-102 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-98 | |||||
1:8 | Average % | 89 | ||||
Range % | 85-96 |
回收率:
The recovery of human ITG/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 89-94 | ||||
EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected502.4392.5692.5042.320251.9331.9871.9601.77612.51.4851.4981.4921.3086.250.9991.0091.0040.8203.120.5750.5970.5860.4021.560.3940.3740.3840.2000.780.3080.3160.3120.12800.1830.1850.184 |
货期:3-5 working days