| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ERp28; ERP29_HUMAN; ERp31; HEL S 107; PDI-DB; PDIA9;;ERp29 |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ERp29 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ERp29抗体的3篇参考文献示例(基于虚构内容，建议核实真实文献)：
1. **"ERp29 regulates the structure and function of the endoplasmic reticulum"**
*作者：Mkrtchian S, Layfield R*
摘要：探讨ERp29作为分子伴侣的结构特征及其在内质网蛋白质折叠与质量控制中的作用，提出其通过二硫键异构酶活性参与分泌蛋白加工。
2. **"ERp29 facilitates protein transport through the secretory pathway"**
*作者：Sargsyan E, et al.*
摘要：研究显示ERp29通过调控COPII囊泡形成促进特定客户蛋白(如甲状腺球蛋白)从内质网向高尔基体的转运，缺失导致分泌受阻。
3. **"ERp29 suppresses breast cancer metastasis via modulating epithelial-mesenchymal transition"**
*作者：Zhang Y, et al.*
摘要：发现ERp29在乳腺癌中低表达，其过表达通过抑制EMT通路(如降低Snail表达)减少癌细胞侵袭，提示其作为转移抑制因子。
4. **"ERp29 deficiency exacerbates α-synuclein toxicity in Parkinson's disease models"**
*作者：Coppola-Segovia V, Stokes MP*
摘要：在帕金森病细胞模型中，ERp29缺失加剧α-突触核蛋白异常聚集和神经元死亡，提示其具有神经保护作用。
**注意**：以上为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。
ERp29 (Endoplasmic Reticulum Protein 29) is a conserved chaperone protein belonging to the protein disulfide isomerase (PDI) family, predominantly localized in the endoplasmic reticulum (ER). Unlike classical PDIs, ERp29 lacks a thioredoxin-like catalytic domain but features a unique dual-domain structure: an N-terminal reductase-like domain and a C-terminal helical domain. It plays a critical role in protein quality control, facilitating the folding, assembly, and secretion of client proteins, particularly those with complex disulfide-bonded structures (e.g., CFTR, lactotransferrin). ERp29 is also implicated in ER stress responses, epithelial-mesenchymal transitions, and cellular differentiation.
Its dysregulation has been linked to cancers, neurodegenerative diseases, and cystic fibrosis. For instance, ERp29 is often downregulated in aggressive tumors, correlating with poor prognosis, while its overexpression may suppress metastasis. In neurodegenerative contexts, ERp29 assists in maintaining proteostasis and mitigating protein aggregation.
ERp29 antibodies are essential tools for detecting and quantifying ERp29 expression in research. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study its localization, expression dynamics, and interactions. Specific antibodies targeting distinct epitopes (e.g., N-terminal vs. C-terminal) enable precise functional studies. Validation via knockout cell lines or siRNA knockdown ensures antibody specificity. Commercial ERp29 antibodies are typically raised in rabbits or mice, with reactivity across human, mouse, and rat samples. These reagents are pivotal in exploring ERp29's roles in health, disease mechanisms, and therapeutic targeting.
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