首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人整合素α-1(ITGA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ITGA1含量。ITGA1是一种细胞表面受体，编码整合素受体的alpha 1亚基，与beta 1亚基结合形成胶原和层粘连蛋白受体，参与细胞间粘附、炎症和纤维化过程。研究显示，ITGA1基因突变与多种疾病相关，如癌症和纤维化，其失调可能通过调节细胞增殖、迁移和组织发育来影响疾病进程。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外检测生物样本中ITGA1的蛋白表达量，可支持肿瘤生物学、炎症机制或纤维化疾病等领域的科学研究，为探讨ITGA1在细胞行为调控及疾病进展中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD 49a ELISA Kit; CD49 antigen-like family member A ELISA Kit; CD49a ELISA Kit; CD49a antigen ELISA Kit; Integrin alpha-1 ELISA Kit; ITA1_HUMAN ELISA Kit; Itga 1 ELISA Kit; ITGA1 ELISA Kit; Laminin and collagen receptor ELISA Kit; Very late activation protein 1 ELISA Kit; VLA 1 ELISA Kit; VLA-1 ELISA Kit; VLA1 ELISA Kit
缩写：ITGA1
Uniprot No.：P56199
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 100 | |||||
| Range % | 100-105 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 87-95 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 94-107 | ||||||
回收率：
| The recovery of human ITGA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 90-101 | |||||
| EDTA plasma (n=4) | 101 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected201.9581.9121.9351.801101.4421.4671.4551.32150.9880.9450.9670.8332.50.5620.5510.5570.4231.250.4160.4230.4200.2860.6250.3030.3090.3060.1720.3120.2360.2310.2340.10000.1330.1350.134 | |||||||
货期：3-5 working days
×
