首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠泛素样蛋白ISG15(ISG15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ISG15含量。ISG15 是一种与干扰素刺激基因相关的蛋白。它在免疫调节、抗病毒防御等方面有重要作用。研究发现，ISG15 可通过与多种蛋白结合（ISGylation 修饰）影响蛋白功能和细胞内信号通路，在肿瘤、感染性疾病等研究中受到关注。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于病毒感染模型、免疫调控机制、细胞应激反应等研究领域。科研人员可通过本产品定量分析小鼠感染病毒后不同组织或体液中的ISG15动态变化，评估干扰素诱导药物对免疫通路的影响，或探究炎症微环境下细胞因子的协同调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Isg15 ELISA Kit; G1p2 ELISA Kit; Ucrp ELISA Kit; Ubiquitin-like protein ISG15 ELISA Kit; Interferon-induced 15 kDa protein ELISA Kit; Interferon-induced 17 kDa protein ELISA Kit; IP17 ELISA Kit; Ubiquitin cross-reactive protein ELISA Kit
缩写：ISG15
Uniprot No.：Q64339
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 106 | ||||
| Range % | 100-112 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of mouse ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 99 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.7342.6272.6812.535102.1462.3062.2262.08051.4291.5021.4661.3202.50.8030.8350.8190.6731.250.4630.4290.4460.3000.6250.3290.3090.3190.1730.3120.2480.2350.2420.09600.1490.1420.146 | ||||||
货期：3-5 working days
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