首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人类泛素蛋白ISG15酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、组织培养上清液样本中的ISG15含量。ISG15是一种与干扰素刺激相关的泛素样蛋白。其背景在于它在机体免疫防御中扮演重要角色。研究机制方面，它可通过共价修饰底物蛋白参与多条信号通路，在抗病毒、抗肿瘤及炎症反应等过程起调控作用，是当前生物医学研究热门靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，该产品特别适用于病毒与宿主互作机制研究、免疫调控网络解析以及炎症相关疾病模型的构建，可为抗病毒药物筛选、免疫信号通路研究及细胞应激反应分析提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：G1P2 ELISA Kit; hUCRP ELISA Kit; IFI 15 ELISA Kit; IFI15 ELISA Kit; Interferon alpha inducible protein ELISA Kit; Interferon induced protein 15 ELISA Kit; Interferon induced protein IFI-15K ELISA Kit; Interferon stimulated protein 15 kDa ELISA Kit; Interferon; alpha-inducible protein (clone IFI-15K) ELISA Kit; Interferon-induced 15 kDa protein ELISA Kit; Interferon-induced 17 kDa protein ELISA Kit; Interferon-induced 17-kDa/15-kDa protein ELISA Kit; IP17 ELISA Kit; ISG 15 ELISA Kit; ISG15 ELISA Kit; ISG15 ubiquitin like modifier ELISA Kit; ISG15_HUMAN ELISA Kit; Ubiquitin cross-reactive protein ELISA Kit; Ubiquitin-like protein ISG15 ELISA Kit; UCRP ELISA Kit
缩写：ISG15
Uniprot No.：P05161
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, cell culture supernates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of human ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-104 | ||||
| EDTA plasma (n=4) | 103 | 98-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.7852.7992.7922.636252.4572.4982.4782.32212.51.9821.9991.9911.8356.251.2721.2911.2821.1263.120.7750.7980.7870.6311.560.5330.5030.5180.3620.780.3310.3560.3440.18800.1550.1570.156 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ISG15 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ISG15 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
×
