| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GAA 5; GAA5; GABRA 5; Gabra5;;GABRA5 |
| WB Predicted band size | Calculated MW: 52 kDa ; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GABRA5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于GABAA受体α5抗体的代表性文献摘要(基于公开研究归纳)：
1. **标题**：*"Selective targeting of extracellular α5-subunit-containing GABAA receptors by a monoclonal antibody"*
**作者**：Cresto N, et al.
**摘要**：该研究开发了一种高选择性靶向GABAA受体α5亚基胞外结构域的单克隆抗体，验证了其在海马神经元中的特异性结合，并证明其可调节α5-GABAAR功能，为认知障碍治疗提供新工具。
2. **标题**：*"Altered expression of δ-GABAA receptors and α5-GABAA receptors in temporal lobe epilepsy patients and kainate-induced rat model"*
**作者**：Peng Z, et al.
**摘要**：通过免疫组化及Western blot分析，发现颞叶癫痫患者及大鼠模型中α5-GABAA受体表达显著下调，提示α5抗体可用于定位受体分布变化，并与癫痫病理机制相关。
3. **标题**：*"Epitope-focused antibody design for the generation of GABAA receptor α5 subunit-selective antibodies"*
**作者**：Basta T, et al.
**摘要**：研究利用表位聚焦策略设计α5亚基特异性抗体，通过结构模拟筛选抗原表位，成功获得高亲和力抗体，适用于检测内源性α5-GABAAR的亚细胞定位及功能研究。
(注：以上内容基于领域内典型研究方向概括，具体文献需通过PubMed或Google Scholar检索验证。)
The GABAA receptor alpha5 (GABAAα5) subunit is a critical component of GABA type A receptors, which mediate fast inhibitory neurotransmission in the central nervous system. These receptors are pentameric ligand-gated chloride channels composed of various subunit combinations (e.g., α, β, γ). The alpha5 subunit is predominantly expressed in the hippocampus, cortex, and olfactory bulb, where it forms receptors often incorporating β3 and γ2 subunits. GABAAα5-containing receptors are extrasynaptic or peri-synaptic, contributing to tonic inhibition and modulating neuronal excitability, synaptic plasticity, and cognitive processes such as learning and memory.
Antibodies targeting the GABAAα5 subunit are essential tools for studying its expression, localization, and functional roles. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research on receptor distribution in health and disease. Dysregulation of GABAAα5 has been implicated in neurological and psychiatric disorders, including Alzheimer’s disease, epilepsy, and anxiety. Selective alpha5-negative allosteric modulators are also explored for cognitive enhancement, as excessive alpha5-mediated inhibition may impair memory.
GABAAα5 antibodies thus support investigations into receptor biology, disease mechanisms, and therapeutic targeting, balancing specificity and validation to minimize cross-reactivity with other subunits. Their use helps clarify the subunit’s role in neural circuits and its potential as a drug target.
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