| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BAP1; DING; BAP-1; HIPI3; RING2; RING1B;;RING2 |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RING2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于RING2/RING1B/RNF2抗体的3篇参考文献，涵盖其功能及抗体应用研究：
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1. **文献名称**：*Polycomb Complexes Repress Developmental Regulators in Murine Embryonic Stem Cells*
**作者**：Boyer, L.A. et al. (2006)
**摘要**：该研究通过ChIP-seq技术结合RING1B抗体，揭示了PRC1复合物(含RING1B/RNF2)在小鼠胚胎干细胞中靶向抑制发育相关基因的机制，证实其在维持多能性中的关键作用。
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2. **文献名称**：*Role of Histone H2A Ubiquitination in Polycomb Silencing*
**作者**：Cao, R. et al. (2002)
**摘要**：文章利用RNF2抗体进行免疫沉淀和功能分析，证明RNF2作为PRC1核心催化亚基，介导组蛋白H2A的K119泛素化，调控基因沉默和胚胎发育过程。
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3. **文献名称**：*A Antibody-Based Characterization of Human Polycomb Repression Complexes*
**作者**：Pengelly, A.R. et al. (2013)
**摘要**：该研究系统性评估了多种Polycomb抗体(包括RING1B抗体)，验证其在免疫印迹(Western blot)和免疫荧光中的特异性，为相关研究提供了抗体选择标准。
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**注**：以上文献均聚焦于RING1B/RNF2的功能及其抗体的实验应用(如ChIP-seq、Western blot等)。如需具体实验细节或更多文献，可进一步检索PubMed或Google Scholar。
The RING2/RING1B/RNF2 antibody targets proteins central to Polycomb group (PcG) complexes, which regulate gene silencing through epigenetic mechanisms. RING1B (also known as RNF2 or RING2) is a core catalytic component of Polycomb Repressive Complex 1 (PRC1). It mediates monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a hallmark of transcriptionally repressive chromatin. This modification facilitates chromatin compaction and collaborates with PRC2-mediated H3K27me3 to maintain long-term gene silencing, critical for cell differentiation, development, and stem cell pluripotency.
Antibodies against RING1B/RNF2 are widely used to study PRC1's role in epigenetic regulation, including its involvement in X-chromosome inactivation, genomic imprinting, and cell cycle control. They are essential tools for detecting protein expression, localization (e.g., nuclear foci), and interaction partners via techniques like Western blot, immunoprecipitation, and immunofluorescence. Dysregulation of RING1B/RNF2 is linked to cancers, developmental disorders, and neurodegenerative diseases, making these antibodies valuable for investigating disease mechanisms. Researchers also use them to explore therapeutic targeting of PRC1 in oncology, as aberrant PcG activity often drives tumor progression. Specificity validation (e.g., knockout cell controls) is crucial due to homology among PcG family members.
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