| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DDX6; HLR2; P54; RCK;;DDX6 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human DDX6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DDX6抗体的3篇参考文献及其摘要概括：
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1. **"Human DDX6 regulates miRNA-mediated gene silencing via direct interactions with the CCR4-NOT complex"**
*Rouya, C., et al. (2014), Nucleic Acids Research.*
摘要：本研究揭示了DDX6通过与CCR4-NOT复合物的直接相互作用，在microRNA介导的基因沉默中起关键作用。利用DDX6抗体进行免疫沉淀实验，证实了其参与翻译抑制和mRNA降解的分子机制。
2. **"DDX6 is required for efficient packaging of murine gammaherpesvirus 68 viral RNA"**
*Ayache, J., et al. (2016), Journal of Virology.*
摘要：研究通过DDX6抗体的免疫荧光和Western blot分析，发现DDX6在病毒RNA包装过程中不可或缺。实验表明，DDX6的缺失会破坏病毒颗粒的形成，提示其在RNA代谢中的广泛功能。
3. **"Structural analysis of the yeast DExH protein Dhh1 reveals a conserved mechanism for coupling RNA remodeling"**
*Mathys, H., et al. (2014), Cell Reports.*
摘要：通过结构生物学和DDX6同源蛋白Dhh1的抗体标记，研究揭示了DDX6家族RNA解旋酶的保守机制，阐明了其如何通过构象变化促进RNA重塑及与其他调控因子的协同作用。
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**备注**：以上文献均涉及DDX6的功能研究，并明确使用了DDX6抗体进行实验验证。如需具体实验细节(如抗体克隆号)，建议查阅原文补充材料或方法部分。
DDX6. also known as Rck/p54. is a conserved RNA helicase belonging to the DEAD-box protein family, characterized by its ATP-dependent RNA unwinding activity. It plays critical roles in various RNA-related processes, including mRNA translation regulation, RNA degradation, and miRNA-mediated gene silencing. DDX6 is a key component of cytoplasmic P-bodies (processing bodies) and stress granules, where it participates in mRNA storage, decay, and post-transcriptional control. Its involvement in repressing translation during stress conditions and modulating RNA-protein interactions makes it essential for cellular adaptation to environmental changes.
Antibodies targeting DDX6 are widely used in molecular and cellular biology to investigate its spatial-temporal expression, subcellular localization, and functional interactions. These antibodies enable techniques like Western blotting, immunofluorescence, immunoprecipitation, and RNA-protein co-localization studies. Research using DDX6 antibodies has revealed its association with viral replication (e.g., hepatitis C virus), cancer progression (via regulation of oncogene expression), and germline development. Commercially available DDX6 antibodies are typically validated for specificity across human, mouse, and rat models, with common epitopes targeting conserved regions like the N-terminal domain. Recent studies employing these antibodies have advanced understanding of DDX6's role in liquid-liquid phase separation dynamics and its potential as a therapeutic target in diseases involving dysregulated RNA metabolism.
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