首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人干扰素调节因子7(IRF7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IRF7含量。IRF7 是一种重要的靶点。它属于干扰素调节因子家族，在天然免疫应答中发挥关键作用。研究表明，IRF7 可激活干扰素基因的表达，增强机体抗病毒能力。其异常表达与多种免疫相关疾病的发生发展机制密切相关，是当前医学研究的热点之一。试剂盒检测范围为23.4 pg/mL-1500 pg/mL，适用于基础医学研究领域，例如探索病毒感染模型中IRF7的表达动态、评估免疫调节药物对IRF7信号通路的影响，或分析不同组织/细胞中IRF7的生物学功能本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IMD39 ELISA Kit; Interferon regulatory factor 7 ELISA Kit; Interferon regulatory factor 7H ELISA Kit; IRF 7 ELISA Kit; IRF 7A ELISA Kit; IRF 7H ELISA Kit; IRF-7 ELISA Kit; IRF7 ELISA Kit; IRF7_HUMAN ELISA Kit; IRF7A ELISA Kit; IRF7B ELISA Kit; IRF7C ELISA Kit; IRF7H ELISA Kit
缩写：IRF7
Uniprot No.：Q92985
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.4 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IRF7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 96-104 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 84-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human IRF7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 94 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 15002.2912.1662.2292.026 7501.9111.8221.8671.664 3751.4471.4561.4521.249 187.51.2001.1771.1890.986 940.8110.8010.8060.603 470.6050.6230.6140.411 23.50.3540.3330.3440.141 00.2050.2010.203 | ||||||
货期：3-5 working days
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