| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EGR alpha; EGRA; Egral; EGRalpha; Gdnfif; KLF10; mGIF; TIEG; TIEG1;;KLF10 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human KLF10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下为3篇关于KLF10抗体的文献示例(模拟构造，供参考)：
1. **文献名称**：*KLF10 regulates TGF-β signaling via modulation of Smad7 expression in hepatocellular carcinoma*
**作者**：Chen L, et al.
**摘要**：研究利用KLF10抗体通过Western blot和免疫组化分析肝癌组织中KLF10蛋白表达，发现KLF10通过抑制Smad7增强TGF-β信号通路，促进肿瘤侵袭。
2. **文献名称**：*Role of KLF10 in cardiac fibrosis revealed by antibody-mediated knockdown models*
**作者**：Wang Y, et al.
**摘要**：通过KLF10特异性抗体进行免疫荧光染色，证实KLF10在心肌成纤维细胞中高表达，并调控胶原沉积，提示其作为心脏纤维化的潜在治疗靶点。
3. **文献名称**：*KLF10 antibody-based detection in diabetic nephropathy reveals podocyte injury mechanisms*
**作者**：Kim S, et al.
**摘要**：采用KLF10抗体对糖尿病肾病肾组织样本进行免疫组化分析，发现KLF10表达上调与足细胞凋亡及蛋白尿进展显著相关，提示其参与氧化应激通路调控。
4. **文献名称**：*KLF10 interacts with p53: Antibody co-immunoprecipitation studies in colorectal cancer*
**作者**：Garcia-Rojas M, et al.
**摘要**：利用KLF10抗体进行Co-IP实验，证明KLF10与p53蛋白直接结合，协同抑制结直肠癌细胞增殖，为靶向治疗提供新依据。
(注：以上文献为模拟示例，实际引用需查询具体数据库如PubMed。)
KLF10 (Krüppel-like factor 10), also known as TIEG1 (TGF-β-inducible early gene-1), is a transcription factor belonging to the Sp/KLF family. It plays critical roles in regulating cell proliferation, differentiation, apoptosis, and extracellular matrix remodeling, primarily through modulation of TGF-β signaling pathways. KLF10 is involved in diverse biological processes, including fibrogenesis, tumorigenesis, metabolism, and osteoblast differentiation. Its expression is induced by TGF-β, steroids, and mechanical stress, and it interacts with other signaling molecules like SMAD proteins to mediate transcriptional responses.
KLF10 antibodies are essential tools for detecting and quantifying KLF10 protein levels in research applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the KLF10 protein. They enable researchers to study KLF10's expression patterns across tissues, its subcellular localization (primarily nuclear), and its dynamic regulation under various pathological conditions. KLF10 dysregulation has been implicated in cancers (e.g., reduced expression in pancreatic cancer, elevated in gliomas), metabolic disorders, and fibrotic diseases, making its antibodies valuable for mechanistic and diagnostic studies. Validation of antibody specificity through knockout controls or siRNA knockdown is crucial due to potential cross-reactivity with other KLF family members.
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