| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 12 LOX; 12LO; 12S LOX; 12S-lipoxygenase; 12S-LOX; Alox12; LOG12; P-12LO;;ALOX12 |
| WB Predicted band size | Calculated MW: 76 kDa ; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human ALOX12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于LOXL2抗体的3篇参考文献(注：LOX12可能为LOXL2的笔误，相关研究集中于肿瘤和纤维化领域)：
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1. **文献名称**: *Anti-LOXL2 Antibody Attenuates Breast Cancer Metastasis*
**作者**: Barry-Hamilton V, et al.
**摘要**: 研究证明LOXL2在乳腺癌转移中通过调节细胞外基质重塑发挥作用。使用特异性LOXL2抗体抑制其活性可显著减少小鼠模型中肿瘤细胞的侵袭和转移，提示其作为治疗靶点的潜力。
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2. **文献名称**: *LOXL2 Expression in Hepatic Fibrosis: A Therapeutic Target*
**作者**: Schilter HC, et al.
**摘要**: 该文献利用LOXL2抗体检测肝纤维化患者组织中LOXL2的过表达，发现其与胶原交联和纤维化进展相关。抗体阻断LOXL2可减轻动物模型的纤维化程度，支持其临床转化价值。
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3. **文献名称**: *Targeting LOXL2 with a Functional Antibody in Pancreatic Cancer*
**作者**: Peng L, et al.
**摘要**: 开发了一种人源化LOXL2抗体，可特异性结合并抑制LOXL2的酶活性。在胰腺癌模型中，该抗体通过阻断肿瘤微环境重塑抑制了肿瘤生长和血管生成。
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**备注**：若确需“LOX12”相关文献，可能需进一步确认目标蛋白名称或研究领域。以上文献可通过PubMed/Google Scholar输入标题或作者姓名查询原文。
**Background of LOX12 Antibody**
The LOX12 antibody is a tool designed to target 12/15-lipoxygenase (12/15-LOX), an enzyme involved in oxidizing polyunsaturated fatty acids, particularly arachidonic acid, to produce bioactive lipid mediators like 12-HETE and 15-HETE. These metabolites play roles in inflammatory signaling, oxidative stress, and cellular homeostasis. The 12/15-LOX pathway is implicated in various pathological conditions, including atherosclerosis, neurodegenerative diseases (e.g., Alzheimer’s), and diabetic complications, where it contributes to tissue damage through pro-inflammatory and pro-oxidant mechanisms.
LOX12 antibodies are widely used in research to detect 12/15-LOX expression and localization in tissues or cells, employing techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help elucidate the enzyme’s role in disease models, such as promoting foam cell formation in atherosclerotic plaques or mediating neuronal oxidative damage. Species reactivity typically includes human, mouse, and rat variants, given the enzyme’s conservation.
Recent studies highlight 12/15-LOX as a potential therapeutic target, driving interest in LOX12 antibodies for validating enzyme inhibition or genetic knockdown in preclinical studies. However, challenges remain in distinguishing its activity from related lipoxygenases (e.g., 5-LOX) due to structural similarities. Overall, LOX12 antibodies serve as critical reagents for advancing understanding of lipid-mediated pathologies and exploring targeted interventions.
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