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Rabbit Monoclonal Cdc27/APC3 Antibody

  • 中文名： Cdc27/APC3抗体
  • 别    名： CDC27Hs; Cell division cycle protein 27 homolog; HNUC; APC3; ANAPC3;;CDC27
货号: IPDX17429
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCDC27Hs; Cell division cycle protein 27 homolog; HNUC; APC3; ANAPC3;;CDC27
WB Predicted band sizeCalculated MW: 92 kDa ; Observed MW: 91 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human CDC27
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

1. **"The anaphase-promoting complex: proteolysis in mitosis and beyond"**

*Authors: Harper, J.W., Burton, J.L., Solomon, M.J.*

摘要：本文综述了后期促进复合体(APC/C)的结构与功能，重点分析了Cdc27/APC3作为核心亚基的作用，并讨论了其在细胞周期调控中的关键性。研究中利用Cdc27抗体进行免疫沉淀实验，验证了APC/C复合体的组装机制。

2. **"Molecular architecture and mechanism of the anaphase-promoting complex"**

*Authors: Peters, J.-M., Zachariae, W.*

摘要：通过冷冻电镜技术解析APC/C的三维结构，确认Cdc27/APC3亚基与其他组分的相互作用位点。Cdc27抗体被用于亚细胞定位实验，揭示了该蛋白在有丝分裂中的动态分布。

3. **"CDC27 homologs and components of the anaphase-promoting complex in diverse eukaryotic species"**

*Authors: Tugendreich, S., et al.*

摘要：跨物种比较研究APC/C的进化保守性，利用Cdc27抗体在酵母和哺乳动物细胞中检测该亚基的表达及功能保守性，证明其在细胞周期调控中的普适性作用。

4. **"Aberrant expression of APC/C subunits in human cancers"**

*Authors: Sudo, T., et al.*

摘要：分析多种癌症组织中APC/C亚基的异常表达，发现Cdc27/APC3在肿瘤细胞中显著下调，并通过Western blot和免疫组化(使用特异性Cdc27抗体)验证其与基因组不稳定的相关性。

背景信息

The Cdc27/APC3 antibody targets a key subunit of the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle progression. APC/C regulates the transition from metaphase to anaphase by tagging specific proteins (e.g., securin, cyclins) with ubiquitin, marking them for proteasomal degradation. Cdc27 (APC3) is a conserved TPR (tetratricopeptide repeat)-containing subunit critical for APC/C assembly and substrate recognition. Antibodies against Cdc27/APC3 are widely used to study cell cycle dynamics, particularly in mitosis and meiosis. They help detect APC/C composition, localization, and activity via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Research using these antibodies has clarified APC/C's role in checkpoint control, genomic stability, and cancer (where dysregulated APC/C activity is linked to tumorigenesis). Cdc27 itself was initially identified in yeast (Cdc27) and later in mammals, with antibodies aiding cross-species functional studies. These tools remain pivotal in exploring how APC/C dysfunction contributes to diseases, drug responses, or developmental defects.

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