首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素11(IL-11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL11含量。IL11是重要靶点。它是一种细胞因子，参与多种生理和病理过程。在研究机制上，IL11与受体结合激活信号通路，影响细胞的增殖、分化等。当前围绕它在肿瘤、纤维化疾病等方面的研究较多，有望为相关疾病治疗带来新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可借助该产品探究IL - 11在炎症性疾病模型、再生医学研究或药物干预实验中的动态变化，为揭示其生物学功能及信号通路机制提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il11 ELISA Kit; Interleukin-11 ELISA Kit; IL-11 ELISA Kit
缩写：IL11
Uniprot No.：Q99MF5
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-94 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-102 | |||||
回收率：
| The recovery of rat IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 80-105 | ||||
| EDTA plasma (n=4) | 98 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.1792.1162.1482.0555001.7731.7041.7391.6462501.4261.3131.3701.2771251.0090.9590.9840.89162.50.5370.5170.5270.43431.20.2620.2500.2560.16315.60.1980.1860.1920.09900.0950.0900.093 | ||||||
货期：3-5 working days
×
