Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL011443HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人Igκ链V-IV区(IGKV4-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物.样本中的IGKV4-1含量。IGKV4 - 1 是重要的靶点。它在相关疾病的发生发展中可能发挥关键作用。研究机制方面，可能涉及到与免疫反应、细胞信号传导等过程的关联，通过对其深入研究，有望揭示疾病的发病机制，为开发针对性的治疗方案奠定基础。试剂盒检测范围为0.156 ng/ml-10 ng/ml，适用于科研领域中对B细胞功能、抗体生成机制及相关疾病模型的探索，例如免疫失调研究、抗体工程开发或肿瘤微环境分析等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
IGKV4-1Immunoglobulin kappa variable 4-1 ELISA Kit; Ig kappa chain V-IV region B17 ELISA Kit; Ig kappa chain V-IV region JI ELISA Kit; Ig kappa chain V-IV region Len ELISA Kit; Ig kappa chain V-IV region STH ELISA Kit
缩写：
IGKV4-1
Uniprot No.：
P06312
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates, cell lysates.
检测范围：
0.156 ng/ml -10 ng/ml
灵敏度：
0.039 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IGKV4-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	96
	Range %	89-103
1:4	Average %	97
	Range %	92-103
1:8	Average %	93
	Range %	86-100

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.069	2.034	2.051	1.922
5	1.347	1.367	1.357	1.228
2.5	0.797	0.828	0.812	0.683
1.25	0.428	0.409	0.419	0.289
0.625	0.294	0.311	0.302	0.173
0.312	0.235	0.243	0.239	0.110
0.156	0.157	0.164	0.160	0.031
0	0.134	0.125	0.130