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产品描述：人Igγ3链C区(IGHG3)酶联免疫试剂盒，为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IGHG3含量。IGHG3（免疫球蛋白重链恒定区3）是一种免疫球蛋白的重链恒定区，参与免疫应答。研究显示，IGHG3在肺结核病患者血清中的含量显著高于健康对照组，可能与肺结核病的发病机制相关。其具体作用机制尚在研究中，但已有研究表明，IGHG3可能作为潜在生物标志物，有助于肺结核病的诊断。试剂盒检测范围为2.7 ng/mL-2000 ng/mL，该产品适用于科研领域中对IgG3亚型表达水平的动态监测，如探索自身免疫疾病病理机制、评估疫苗免疫效果、研究免疫调节药物干预后的体液免疫变化等，为免疫学研究和生物标志物发现提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGHG3Immunoglobulin heavy constant gamma 3 ELISA Kit; HDC ELISA Kit; Heavy chain disease protein ELISA Kit; Ig gamma-3 chain C region ELISA Kit
缩写：IGHG3
Uniprot No.：P01860
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：2.7 ng/mL-2000 ng/mL
灵敏度：2.7 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGHG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 99 | ||||
| Range % | 93-106 | |||||
| 1:2000 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4000 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human IGHG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average20000.1730.1720.17256670.2590.2460.25252220.3670.3440.3555740.4880.4590.4735250.6330.6240.62858.20.7890.8550.8222.71.0291.0331.03101.6971.7481.7225 | ||||||
货期：3-5 working days
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