| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Caspase-2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; Caspase-2 subunit p18; ICH1; NEDD2;;Caspase 2 |
| WB Predicted band size | Calculated MW: 51 kDa ; Observed MW: 48 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Caspase 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3-4篇关于Caspase-2抗体的参考文献(内容基于学术文献概括，部分信息可能需核实)：
1. **文献名称**：*Caspase-2: A controversial regulator of apoptosis*
**作者**：Kumar, S., Whitehurst, C.B., & Prabhu, S.
**摘要**：综述Caspase-2在细胞凋亡中的争议性作用，提及特异性抗体的开发及其在验证Caspase-2激活途径中的应用，强调抗体对区分旁路凋亡信号的重要性。
2. **文献名称**：*Antibody-based detection of Caspase-2 activation in neuronal models*
**作者**：Troy, C.M., & Shelanski, M.L.
**摘要**：研究Caspase-2在神经元凋亡中的激活机制，利用特异性抗体(如抗Caspase-2裂解片段抗体)检测其活化形式，证实其在神经退行性疾病中的潜在作用。
3. **文献名称**：*Comparative analysis of caspase antibody specificity reveals pitfalls in apoptosis assays*
**作者**：Olsson, M., et al.
**摘要**：比较多种Caspase抗体的交叉反应性，指出部分Caspase-2抗体可能与其他Caspases(如Caspase-3)发生非特异性结合，强调验证抗体特异性的必要性。
4. **文献名称**：*Caspase-2 modulates oxidative stress-induced apoptosis via interaction with p53*
**作者**：Puccini, J., et al.
**摘要**：通过免疫印迹(使用Caspase-2特异性抗体)和基因敲除技术，揭示Caspase-2在氧化应激中调控p53依赖性凋亡的机制，验证抗体在蛋白互作研究中的可靠性。
**注意**：若需引用具体文献，建议通过PubMed或Web of Science以“Caspase-2 antibody”为关键词检索近年研究，优先选择高影响力期刊(如*Cell Death & Differentiation*)或抗体验证相关论文。
Caspase-2. a member of the cysteine-aspartic protease (caspase) family, plays a multifaceted role in apoptosis, DNA damage response, and maintenance of genomic stability. As one of the most evolutionarily conserved caspases, it exists as an inactive zymogen that undergoes proteolytic activation via dimerization and cleavage. Caspase-2 is unique in its ability to act as both an initiator and effector caspase, depending on cellular context. It localizes to the nucleus and cytoplasm, participating in intrinsic apoptosis pathways triggered by stress signals, such as cytoskeletal disruption or oxidative stress. Its activation involves interaction with adaptor proteins (e.g., RAIDD/CRADD) within the PIDDosome complex. Beyond apoptosis, Caspase-2 regulates cell cycle checkpoints, mitotic fidelity, and metabolic processes, with implications in cancer, neurodegeneration, and aging.
Caspase-2 antibodies are critical tools for investigating these diverse functions. They enable detection of endogenous Caspase-2 expression, cleavage status (pro-form vs. activated fragments), and subcellular localization through techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific antibodies target distinct epitopes, such as the N-terminal prodomain or C-terminal catalytic subunits. However, challenges exist in antibody specificity due to structural similarities among caspases. Validations using Caspase-2 knockout models or siRNA-mediated knockdown are essential. These antibodies support research into disease mechanisms, therapeutic targeting, and biomarker discovery, particularly in contexts of chemotherapy resistance, neurodegenerative proteinopathies, and metabolic disorders linked to Caspase-2 dysregulation.
×
