| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | aPKCzeta; nPKC zeta; PKC 2; PKC ZETA; PKCZETA ; Protein kinase C zeta; r14-3-3;;p-PKC zeta (T560) |
| WB Predicted band size | 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKC zeta around the phosphorylation site of T560 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及Phospho-PKCζ(T560)抗体的参考文献概览：
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1. **文献名称**：*PKCζ Phosphorylation at Thr560 Regulates NF-κB Signaling in Mast Cells*
**作者**：Leitges M. et al.
**摘要**：研究了PKCζ Thr560位点的磷酸化在肥大细胞NF-κB激活中的作用，通过Phospho-PKCζ(T560)抗体证实该位点的磷酸化是IKK复合体活化的关键步骤，影响炎症反应。
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2. **文献名称**：*Phosphorylation of PKCζ at Thr560 Modulates Intestinal Epithelial Polarity*
**作者**：Suzuki A. & Ohno S.
**摘要**：利用Phospho-PKCζ(T560)特异性抗体，发现该位点的磷酸化通过调节Par3复合体影响肠上皮细胞极性建立，揭示其在组织形态发生中的机制。
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3. **文献名称**：*Role of PKCζ T560 Phosphorylation in Cancer Cell Invasion*
**作者**：Fields A.P. et al.
**摘要**：通过Western blot和免疫组化分析，证明肿瘤细胞中PKCζ Thr560的磷酸化水平升高与侵袭性相关，该抗体用于评估临床样本中PKCζ激活状态。
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**备注**：若需具体文献来源，建议通过PubMed或抗体供应商(如CST #9378)的产品引用列表进一步检索。部分研究可能需结合全文确认抗体使用细节。
The Phospho-PKCζ (Thr560) antibody detects the activated form of Protein Kinase C zeta (PKCζ), a member of the atypical PKC subfamily. Unlike conventional PKC isoforms, PKCζ does not require calcium or diacylglycerol for activation but relies on phosphorylation for its enzymatic activity. Thr560. located in the activation loop of the kinase domain, is a critical phosphorylation site required for PKCζ activation. This phosphorylation event, mediated by upstream kinases such as PDK1 (3-phosphoinositide-dependent protein kinase 1), stabilizes the active conformation of PKCζ, enabling its participation in downstream signaling pathways.
PKCζ plays a central role in cell survival, polarity, and inflammatory responses, often interacting with components of the PI3K/Akt/mTOR and NF-κB pathways. Dysregulation of PKCζ activity has been implicated in cancer, insulin resistance, and neurodegenerative disorders. The Phospho-PKCζ (Thr560) antibody is widely used in research to study PKCζ activation dynamics under conditions such as growth factor stimulation, oxidative stress, or metabolic challenges. It is validated for applications including Western blotting, immunofluorescence, and immunohistochemistry, helping to elucidate PKCζ's context-dependent roles in health and disease. Specificity is confirmed using knockout controls or peptide competition assays, ensuring accurate detection of the phosphorylated form without cross-reactivity to other PKC isoforms.
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