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产品描述：人Ig α1链C区(IGHA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IGHA1含量。IGHA1作为靶点，在相关研究中有重要价值。它可能与特定生理病理机制有关，研究主要聚焦于其在疾病发生发展里的作用机制，探索以它为靶点开发针对性药物或治疗手段，有望为相关疾病的防治带来新突破。试剂盒检测范围为0.49 ng/mL-2000 ng/mL，适用于免疫学基础研究、黏膜相关疾病机制探索（如感染性疾病或过敏模型）、免疫缺陷评估等科研领域。实验样本兼容常规生物液体样本类型，尤其适合评估体液免疫应答动态或特定病理条件下IgA抗体的表达特征，为研究黏膜免疫调控、宿主 - 病原体互作等课题提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGHA1 ELISA Kit; Immunoglobulin heavy constant alpha 1 ELISA Kit; Ig alpha-1 chain C region ELISA Kit; Ig alpha-1 chain C region BUR ELISA Kit; Ig alpha-1 chain C region TRO ELISA Kit
缩写：IGHA1
Uniprot No.：P01876
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.49 ng/mL-2000 ng/mL
灵敏度：0.49 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGHA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5000 | Average % | 92 | |||||
| Range % | 85-103 | ||||||
| 1:10000 | Average % | 91 | |||||
| Range % | 85-99 | ||||||
| 1:20000 | Average % | 97 | |||||
| Range % | 90-108 | ||||||
| 1:40000 | Average % | 92 | |||||
| Range % | 83-100 | ||||||
回收率：
| The recovery of human IGHA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 83-101 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 20002.2342.2852.2602.171 5002.1122.1652.1392.050 1251.8861.8971.8921.803 31.251.2651.2451.2551.166 7.810.6080.5870.5980.509 1.950.2900.2860.2880.199 0.490.1360.1390.1380.049 00.0860.0910.089 | |||||||
货期：3-5 working days
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