| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DOT 1; DOT1L; KMT4; Histone methyltransferase DOT1L; H3 lysine-79 specific; ;KMT4 |
| WB Predicted band size | Calculated MW: 165 kDa ; Observed MW: 185 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human KMT4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于KMT4/Dot1L抗体的3篇代表性文献，简要整理如下：
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1. **文献名称**：*"DOT1L inhibits SIRT1-mediated epigenetic silencing to maintain leukemic gene expression in MLL-rearranged leukemia"*
**作者**：Muellers, E. et al.
**摘要**：该研究利用Dot1L特异性抗体进行ChIP-seq分析，揭示了Dot1L通过催化H3K79甲基化，拮抗SIRT1介导的基因沉默，维持MLL白血病相关致癌基因表达的机制。抗体的应用证实了Dot1L在染色质结合中的功能。
2. **文献名称**：*"Structural basis for DOT1L interaction with the AF10 oligomerization domain"*
**作者**：McGinty, R.K. et al.
**摘要**：本文通过免疫共沉淀(Co-IP)和蛋白质互作分析，使用Dot1L抗体阐明了Dot1L与AF10蛋白的结构互作机制，揭示了其在组蛋白甲基化复合体组装中的关键作用。
3. **文献名称**：*"DOT1L regulates stress-dependent gene transcription through H3K79 methylation"*
**作者**：Barry, E.R. et al.
**摘要**：研究通过Western blot和免疫荧光技术，利用Dot1L抗体证明其在细胞应激反应中调控H3K79me2水平，并影响下游基因转录的功能，为Dot1L在表观遗传应激应答中的作用提供证据。
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这些文献均通过Dot1L抗体在染色质分析、蛋白互作验证及功能研究中展开探索，支撑了其在表观遗传调控中的关键角色。如需更多文献，可进一步检索PubMed或SciHub获取全文。
**Background of KMT4/Dot1L Antibody**
The KMT4 (lysine methyltransferase 4) protein, also known as Dot1L (Disruptor of Telomeric Silencing 1-like), is a conserved histone methyltransferase that catalyzes the methylation of histone H3 on lysine 79 (H3K79). Unlike many histone-modifying enzymes, Dot1L methylates the globular core of histone H3 rather than the N-terminal tail, a unique mechanism linked to transcriptional regulation, DNA repair, and cell cycle progression. Dot1L-mediated H3K79 methylation (mono-, di-, or tri-methylation) is associated with active chromatin regions and plays roles in leukemogenesis, development, and cellular differentiation.
Antibodies targeting Dot1L/KMT4 are critical tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate Dot1L's involvement in epigenetic regulation and disease. Dysregulation of Dot1L is linked to mixed-lineage leukemia (MLL) and other cancers, making these antibodies valuable for both basic research and therapeutic development. Specific Dot1L antibodies are validated for species cross-reactivity, isoform specificity, and application compatibility to ensure accurate detection in experimental models.
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