| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor 7-like 1; HMG box transcription factor 3; TCF-3; TCF7L1; TCF3;;TCF7L1 |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human TCF7L1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TCF3抗体的3篇代表性文献示例(注：以下内容为示例性概括，实际文献需通过学术数据库查询)：
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1. **文献名称**: *TCF3 mutations in B-cell acute lymphoblastic leukemia: mechanisms of transcriptional deregulation and therapeutic implications*
**作者**: Mendes A, et al.
**摘要**: 本研究利用TCF3特异性抗体进行免疫沉淀和染色质免疫共沉淀(ChIP)实验，揭示了TCF3基因突变导致B细胞白血病中转录调控异常，并发现其与PAX5等转录因子的协同作用失调。抗体应用包括Western blot和免疫组化分析患者样本。
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2. **文献名称**: *E2A proteins maintain pluripotency of embryonic stem cells through direct regulation of Nanog*
**作者**: Pereira CF, et al.
**摘要**: 通过TCF3/E2A抗体进行染色质分析和免疫荧光实验，研究发现TCF3通过直接结合Nanog启动子维持胚胎干细胞多能性。抗体在敲除模型中验证了蛋白表达缺失对干细胞分化的影响。
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3. **文献名称**: *The role of TCF3 in lymphoid development: Insights from antibody-mediated protein interaction mapping*
**作者**: Smith J, et al.
**摘要**: 使用TCF3抗体进行免疫共沉淀和质谱分析，揭示了TCF3在B细胞分化过程中与E蛋白家族成员(如HEB和E2-2)形成异源二聚体，调控靶基因如EBF1的转录活性，抗体特异性验证通过siRNA敲降实验完成。
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如需具体文献，建议在PubMed或Google Scholar中搜索关键词“TCF3 antibody”、“E2A immunoprecipitation”等，并筛选近年高被引研究。
TCF3 (Transcription Factor 3), also known as E2A, is a member of the E-protein family of basic helix-loop-helix (bHLH) transcription factors encoded by the *TCF3* gene. It plays a critical role in regulating gene expression during cellular differentiation, particularly in B-cell and T-cell development within the immune system. TCF3 binds to E-box DNA motifs and activates or represses target genes involved in lymphocyte maturation, including those necessary for V(D)J recombination during immunoglobulin and T-cell receptor formation. It also interacts with regulatory proteins like ID (Inhibitor of DNA-binding) to modulate cell cycle progression and differentiation.
Dysregulation of TCF3 is implicated in several pathologies. Chromosomal translocations involving *TCF3*, such as t(1;19) in acute lymphoblastic leukemia (ALL), generate fusion proteins (e.g., E2A-PBX1) that disrupt normal transcriptional programs, promoting oncogenesis. Additionally, altered TCF3 activity is linked to immune disorders and some solid tumors.
TCF3 antibodies are essential tools for studying its expression, localization, and interactions in both normal and diseased states. They are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its role in development and disease. Commercial TCF3 antibodies are often validated for specificity against epitopes within its N-terminal activation domains or C-terminal bHLH region, aiding research in immunology, hematopoiesis, and cancer biology.
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