| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cadherin;CDH3;CDHP;Cadherin-1;Cadherin-2;Cadherin-3;Cadherin-4;Cadherin-5;CAM 120/80;CD144;CD324;CD325;Cdh4;CDH5;CDHE;CDHN;E-cadherin;N-cadherin;NCAD;Neural cadherin;P-cadherin;Uvomorulin;Vascular endothelial cadherin ;Cadherin |
| WB Predicted band size | Calculated MW: 15-20,80-83 kDa ; Observed MW: 140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Cadherin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于pan-Cadherin抗体的3篇参考文献，包含名称、作者及摘要概括：
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1. **"Pan-Cadherin Antibody Characterization in Epithelial-Mesenchymal Transition Studies"**
*Author: Thompson, R. et al.*
摘要：该研究验证了一种广谱pan-Cadherin抗体在检测多种Cadherin亚型(如E-、N-和P-Cadherin)中的交叉反应性，应用于乳腺癌细胞系和肿瘤组织切片，证明其在EMT(上皮-间质转化)研究中的可靠性。
2. **"Utility of pan-Cadherin as a Marker for Cellular Adhesion in Developmental Biology"**
*Author: Suzuki, K. & Watanabe, T.*
摘要：通过免疫组化和Western blot，评估pan-Cadherin抗体在小鼠胚胎发育过程中细胞黏附结构的定位，发现其在多种组织(如神经管、体节)中广泛表达，支持其在发育动态研究中的应用。
3. **"Comparative Analysis of Cadherin Expression Using pan-Cadherin Antibodies in Human Carcinomas"**
*Author: García, M. et al.*
摘要：研究比较了pan-Cadherin抗体与特异性Cadherin抗体在结直肠癌和肺癌中的表达模式，证实pan-Cadherin可作为初步筛查工具，但需结合亚型特异性抗体进行精准分析。
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以上文献涵盖抗体验证、发育生物学及癌症研究中的典型应用，为pan-Cadherin抗体的多功能性提供参考。如需具体期刊或年份，可进一步补充检索条件。
Pan-Cadherin antibodies are immunological tools designed to recognize a broad spectrum of cadherin family members, which are calcium-dependent transmembrane glycoproteins critical for cell-cell adhesion and tissue morphogenesis. Cadherins share a conserved extracellular domain with repeated cadherin motifs (EC domains) and a cytoplasmic domain that interacts with catenins to link the cytoskeleton. The term "pan" indicates these antibodies target conserved epitopes across multiple cadherin subtypes (e.g., E-, N-, P-cadherin), making them useful for detecting classical cadherins without subtype specificity.
These antibodies are widely employed in research to study cell adhesion dynamics, epithelial-mesenchymal transition (EMT), and tissue integrity in development, cancer metastasis, and wound healing. For example, pan-Cadherin antibodies can identify cadherin expression shifts during tumor progression or embryonic patterning. They are commonly used in techniques like immunohistochemistry, Western blotting, and immunofluorescence.
However, their broad reactivity may limit subtype-specific analyses, necessitating validation with isoform-specific antibodies for precise studies. Commercially available pan-Cadherin antibodies are often raised against conserved cytoplasmic regions (e.g., amino acid residues near the C-terminus) of human or murine cadherins, with cross-reactivity verified in multiple species. Researchers must optimize conditions to balance sensitivity and specificity, particularly in tissues with mixed cadherin expression. Overall, pan-Cadherin antibodies remain indispensable for exploring cadherin-mediated adhesion in diverse biological contexts.
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