| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CRADD;MGC9163;RAIDD;Death adaptor molecule RAIDD;Death domain containing protein CRADD;;CRADD |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human CRADD |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于RAIDD抗体的3篇经典文献及其摘要：
---
1. **文献名称**：*RAIDD is a new death adaptor molecule*
**作者**：Duan, H., Dixit, V.M.
**摘要**：该研究首次鉴定了RAIDD蛋白，揭示了其通过死亡结构域(death domain)介导Caspase-2激活的机制。文献中提到使用特异性RAIDD抗体验证其与RIP(Receptor-interacting protein)及Caspase-2的相互作用，为凋亡信号通路研究奠定基础(*Nature*, 1997)。
---
2. **文献名称**：*The PIDDosome, a protein complex implicated in activation of Caspase-2 in response to genotoxic stress*
**作者**：Hsu, H., et al.
**摘要**：研究聚焦于PIDDosome复合体(PIDD-RAIDD-Caspase-2)，发现RAIDD在DNA损伤后促进Caspase-2激活。通过RAIDD抗体进行免疫共沉淀实验，证实了复合体的形成及其在凋亡中的作用(*Science*, 2004)。
---
3. **文献名称**：*Caspase-2 induces apoptosis via the PIDDosome-mediated pathway*
**作者**：Tinel, A., Tschopp, J.
**摘要**：本文阐明了Caspase-2依赖PIDDosome复合体激活的分子机制。利用RAIDD特异性抗体阻断复合体组装，证实RAIDD在DNA损伤诱导的凋亡中不可或缺(*Cell*, 2004)。
---
**备注**：上述文献均涉及RAIDD抗体的实验应用(如Western blot、免疫共沉淀)，但部分研究可能未直接以RAIDD抗体为核心，而是作为工具验证其功能。如需更针对性文献，建议通过PubMed或Google Scholar检索关键词“RAIDD antibody”。
RAIDD (RIP-associated ICH-1/CED-3 homologous protein with a death domain) is an adaptor protein involved in apoptosis and inflammatory signaling pathways. It contains a C-terminal death domain (DD) and an N-terminal caspase recruitment domain (CARD), enabling interactions with other death domain-containing proteins like RIPK1 and caspase-2. RAIDD plays a critical role in assembling the PIDDosome complex (composed of PIDD, RAIDD, and caspase-2), which activates caspase-2 in response to DNA damage or cellular stress. This caspase-2 activation triggers downstream apoptotic cascades or NF-κB-mediated inflammatory responses, depending on cellular context.
RAIDD antibodies are essential tools for studying its expression, localization, and protein interactions in apoptosis regulation. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate RAIDD's role in diseases like cancer, neurodegenerative disorders, and autoimmune conditions. Some studies suggest RAIDD may act as a tumor suppressor by promoting apoptosis, while others implicate it in neuroinflammation pathways. Commercial RAIDD antibodies are typically raised against specific epitopes within its DD or CARD regions, with validation in knockout models to ensure specificity. Researchers also utilize these antibodies to explore post-translational modifications or alternative splicing variants of RAIDD that may influence its pro-apoptotic functions. Optimal antibody selection depends on experimental applications, as some isoforms or truncated forms may exhibit tissue-specific expression.
×
