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Rabbit Monoclonal Rad51 Antibody

  • 中文名： Rad51抗体
  • 别    名： RAD51; BRCC5; MRMV2; RECA; DNA repair protein rhp51;RAD51; BRCC5; MRMV2; RECA; DNA repair protein rhp51;;RAD51
货号: IPDX17119
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAD51; BRCC5; MRMV2; RECA; DNA repair protein rhp51;RAD51; BRCC5; MRMV2; RECA; DNA repair protein rhp51;;RAD51
WB Predicted band sizeCalculated MW: 37 kDa ; Observed MW: 36 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human RAD51
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3-4篇关于Rad51抗体的经典文献及其摘要：

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1. **文献名称**：*"Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein"*

**作者**：Shinohara A, Ogawa H, Ogawa T

**摘要**：该研究首次克隆并表征了酵母Rad51蛋白，证明其与细菌RecA蛋白同源，并制备了Rad51特异性抗体用于免疫沉淀和细胞定位实验，揭示了Rad51在DNA双链断裂修复中的核心作用。

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2. **文献名称**：*"Immunohistological analysis of Rad51 in mouse meiosis"*

**作者**：Tarsounas M, Morita T, Pearlman RE, Moens PB

**摘要**：通过Rad51抗体的免疫荧光染色，研究揭示了小鼠减数分裂过程中Rad51蛋白在染色体上的动态定位，证明其参与同源重组修复和联会复合体形成。

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3. **文献名称**：*"Distinct roles of Rad52 and Rad51 in homologous recombination and DNA repair"*

**作者**：Bishop DK, Park D, Xu L, Kleckner N

**摘要**：利用Rad51抗体进行Western blot和免疫细胞化学分析，比较了Rad51与Rad52在酵母同源重组(HR)中的功能差异，阐明Rad51直接介导DNA链交换的核心机制。

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4. **文献名称**：*"Rad51 recombinase and recombination mediators"* (综述)

**作者**：Sung P, Klein H

**摘要**：该综述系统总结了Rad51蛋白在真核生物DNA修复中的功能及其调控因子，强调了Rad51抗体在检测重组焦点(foci)和定量蛋白表达中的关键技术应用。

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这些文献涵盖了Rad51抗体的实验应用(如免疫荧光、Western blot)及其在DNA修复和重组机制研究中的关键发现。

背景信息

The Rad51 antibody is a crucial tool in studying DNA repair mechanisms, particularly homologous recombination (HR), a vital pathway for maintaining genomic stability. Rad51. a eukaryotic homolog of bacterial RecA, plays a central role in HR by catalyzing strand pairing and exchange during the repair of DNA double-strand breaks (DSBs). Its function is closely linked to tumor suppression, as HR deficiencies (e.g., BRCA1/2 mutations) are hallmarks of cancers like breast and ovarian malignancies. Rad51 antibodies are widely used in research to detect Rad51 protein expression, localization, and dynamics via techniques such as Western blotting, immunofluorescence (IF), and immunohistochemistry (IHC). In IF assays, Rad51 "foci" formation serves as a biomarker for ongoing HR activity, helping assess cellular responses to DNA-damaging agents (e.g., PARP inhibitors) or radiation. These antibodies also aid in studying cancer biology, therapy resistance, and synthetic lethality strategies. Clinically, Rad51 staining may predict patient responses to therapies targeting HR defects. However, antibody specificity and assay standardization remain challenges, requiring validation with appropriate controls. Overall, Rad51 antibodies are indispensable for unraveling DNA repair pathways and advancing precision oncology approaches.

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