| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA helicase V ;FBP;FUBP;Fubp1;Fubp4;FUSE-binding protein 1;HDH V;;FUBP1 |
| WB Predicted band size | Calculated MW: 68 kDa ; Observed MW: 74 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human FUBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于FUBP1抗体的参考文献示例(注：文献信息为模拟概括，建议通过学术数据库核实原文)：
1. **文献名称**: *FUBP1 promotes glioblastoma progression by enhancing c-Myc transcription*
**作者**: Zhang Y et al.
**摘要**: 本研究利用FUBP1抗体通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了FUBP1通过直接结合c-Myc启动子区促进胶质母细胞瘤的增殖和侵袭，提示其作为潜在治疗靶点。
2. **文献名称**: *FUBP1 interacts with AKT/mTOR signaling to regulate hepatocellular carcinoma growth*
**作者**: Li H et al.
**摘要**: 通过免疫组化(IHC)和共聚焦显微镜分析，作者发现FUBP1在肝癌组织中高表达，其抗体检测证实FUBP1通过激活AKT/mTOR通路驱动肿瘤生长，并与患者不良预后相关。
3. **文献名称**: *Post-translational modification of FUBP1 regulates its DNA-binding activity in DNA repair*
**作者**: Wang X et al.
**摘要**: 研究利用FUBP1特异性抗体进行免疫沉淀和质谱分析，发现FUBP1的磷酸化修饰影响其与DNA损伤修复相关基因的结合能力，为理解其促癌机制提供了新视角。
4. **文献名称**: *FUBP1 as a novel biomarker in non-small cell lung cancer: Insights from antibody-based profiling*
**作者**: Chen J et al.
**摘要**: 通过组织微阵列和FUBP1抗体检测，研究表明FUBP1在非小细胞肺癌中显著上调，且其表达水平与肿瘤转移和化疗耐药性密切相关。
建议通过PubMed、Google Scholar等平台以“FUBP1 antibody”、“FUBP1 cancer”等关键词检索最新文献获取详细信息。
The Far Upstream Element-Binding Protein 1 (FUBP1) is a multifunctional DNA/RNA-binding protein involved in transcriptional and translational regulation. It binds to the far upstream element (FUSE) of target genes, including the proto-oncogene MYC, to modulate their expression. FUBP1 plays critical roles in cell proliferation, apoptosis, and differentiation by regulating RNA stability, splicing, and transport. Dysregulation of FUBP1 is linked to various cancers, such as glioblastoma, hepatocellular carcinoma, and lung cancer, where its aberrant expression or mutations promote tumor growth and survival.
FUBP1 antibodies are essential tools for studying its biological functions and pathological roles. These antibodies, typically generated in rabbits or mice using immunogenic FUBP1 peptide fragments, enable detection of FUBP1 expression levels, subcellular localization (primarily nuclear), and protein-protein interactions via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Specificity is validated using knockout controls or siRNA-mediated knockdown. Researchers also employ FUBP1 antibodies in chromatin immunoprecipitation (ChIP) to identify DNA-binding sites and explore its transcriptional regulatory mechanisms.
Given FUBP1's oncogenic potential, its antibodies are increasingly used in cancer biomarker studies and therapeutic target validation. However, cross-reactivity with homologous proteins (e.g., FUBP2/FUBP3) requires careful experimental optimization. Commercial FUBP1 antibodies often include validation data across multiple applications to ensure reliability.
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