| WB | 咨询技术 | Human,Mouse,Rat,Cow,Dog |
| IF | 1/20-1/50 | Human,Mouse,Rat,Cow,Dog |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat,Cow,Dog |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Cow,Dog |
| FCM | 咨询技术 | Human,Mouse,Rat,Cow,Dog |
| Elisa | 咨询技术 | Human,Mouse,Rat,Cow,Dog |
| Aliases | GM130; Gm130 autoantigen; GOLGA 2; Golga2; Golgi autoantigen; Golgin 95;;Golgin subfamily A member 2 |
| WB Predicted band size | Calculated MW: 113 kDa ; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey,Cow,Dog |
| Immunogen | A synthesized peptide derived from human Golgin subfamily A member 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GM130抗体的3篇参考文献的简要概括：
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1. **文献名称**：*GM130. a cis-Golgi protein, is a member of the SNAP receptor complex*
**作者**：Nakamura N, et al.
**摘要**：该研究通过GM130抗体的免疫沉淀分析，揭示了GM130作为顺面高尔基体基质蛋白，参与维持高尔基体结构的完整性，并与SNAP受体复合物相互作用，调控囊泡运输过程。
2. **文献名称**：*The Golgi matrix protein GM130: a specific marker of interphase Golgi in mammalian cells*
**作者**：Barr FA, et al.
**摘要**：作者利用GM130抗体进行免疫荧光和电镜研究，证实GM130在细胞间期特异性标记高尔基体，并在有丝分裂期间随高尔基体解体而分散，为研究高尔基体动态提供了关键工具。
3. **文献名称**：*GRASP65 and GM130 synergistically regulate Golgi morphology and trafficking*
**作者**：Shorter J, et al.
**摘要**：通过GM130抗体的功能抑制实验，研究发现GM130与GRASP65协同调控高尔基体叠层形成，并影响蛋白质分泌途径，强调了其在细胞膜系统组织中的核心作用。
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以上文献均以GM130抗体为核心实验工具，探讨了其在细胞器结构、运输及分裂中的功能。如需具体年份或期刊信息，可进一步补充检索。
GM130. also known as Golgin subfamily A member 2 (GOLGA2), is a 130 kDa Golgi matrix protein encoded by the *GOLGA2* gene in humans. It is predominantly localized to the cis-Golgi network and plays a critical role in maintaining Golgi structure, vesicle trafficking, and glycosylation processes. GM130 interacts with other Golgi matrix proteins, such as GRASP65. to form the Golgi ribbon and ensure proper organelle stacking. Additionally, it participates in cell cycle regulation by contributing to Golgi disassembly and reassembly during mitosis.
GM130 antibodies are widely used tools in cell biology research to study Golgi morphology, dynamics, and functional disruptions. These antibodies, often raised in rabbits or mice, specifically recognize GM130 epitopes in techniques like immunofluorescence, Western blotting, and immunohistochemistry. Researchers rely on GM130 as a cis-Golgi marker to assess Golgi integrity in experimental models, including cancer, neurodegenerative diseases, and infectious diseases where Golgi fragmentation or dysfunction occurs. For example, altered GM130 expression has been linked to impaired protein trafficking in Alzheimer’s disease and disrupted cell division in certain cancers. When using GM130 antibodies, co-staining with other Golgi markers (e.g., TGN46 or Giantin) is recommended to validate subcellular localization and experimental outcomes.
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