| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H3.1; Histone H3; HIST1H3A; H3K4me2;;DiMethyl-Histone H3 (K5) |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the methylation site of K5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于 **Methyl-Histone H3 (diK4)**(即 **H3K4me2**)抗体的3篇参考文献，涵盖其应用及功能研究：
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1. **文献名称**：*"High-resolution profiling of histone methylations in the human genome"*
**作者**：Barski, A., Cuddapah, S., Cui, K., Roh, T.Y., Schones, D.E., Wang, Z., et al.
**摘要**：利用 **H3K4me2 抗体**进行 ChIP-seq 分析，揭示其在基因启动子及增强子区域的富集，关联于转录激活和表观遗传调控，为组蛋白修饰图谱研究提供方法学基础(*Nature*, 2007)。
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2. **文献名称**：*"Histone methylation regulates gene expression during differentiation and cancer"*
**作者**：Bernstein, B.E., Mikkelsen, T.S., Xie, X., et al.
**摘要**：通过 **H3K4me2 抗体**的染色质免疫沉淀技术，发现其在小鼠胚胎干细胞分化过程中标记活跃的增强子区域，证实其在细胞命运决定中的关键作用(*Cell*, 2006)。
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3. **文献名称**：*"Global mapping of histone modifications in human cells using tiling microarrays"*
**作者**：Kim, T.H., Barrera, L.O., Zheng, M., et al.
**摘要**：结合 **H3K4me2 抗体**和高通量芯片技术，绘制人类基因组组蛋白修饰图谱，显示其与转录起始位点的强相关性，为表观遗传调控机制提供证据(*Nature Genetics*, 2005)。
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**备注**：若需具体实验步骤或抗体验证细节，可参考厂商(如Abcam、Cell Signaling Technology)提供的技术文档或应用指南。
Methyl-Histone H3 (diK4) antibodies are essential tools for studying epigenetic regulation, specifically targeting histone H3 proteins dimethylated at lysine 4 (H3K4me2). Histone H3 is a core component of nucleosomes, and post-translational modifications, such as methylation, play a critical role in chromatin structure and gene expression. H3K4 methylation is generally associated with active transcription, with different methylation states (mono-, di-, or trimethylation) correlating with distinct regulatory outcomes. Dimethylation at H3K4 (H3K4me2) is often found at enhancers and promoter-proximal regions, marking transcriptionally poised or active genes.
These antibodies are widely used in chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map genomic regions associated with transcriptional activation or epigenetic memory. Researchers employ them to investigate roles in development, differentiation, and diseases like cancer, where aberrant H3K4 methylation patterns are linked to oncogenesis. Specificity is critical, as cross-reactivity with other methylated histone residues (e.g., H3K9 or H3K27) or methylation states (me1/me3) can lead to misinterpretation. Validation using knockout cells or peptide competition assays is recommended.
Overall, Methyl-Histone H3 (diK4) antibodies are indispensable for decoding the dynamic interplay between histone modifications and cellular function, advancing our understanding of epigenetic mechanisms in health and disease.
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