| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PENT; PNMT; PNMTase;;PNMTase |
| WB Predicted band size | Calculated MW: 31 kDa ; Observed MW: 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human PNMTase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PNMT(苯乙醇胺-N-甲基转移酶)抗体的3篇参考文献及其简要摘要：
1. **《Production and characterization of monoclonal antibodies to human phenylethanolamine N-methyltransferase》**
- **作者**：Kennedy B. et al.
- **摘要**：该研究报道了针对人PNMT的单克隆抗体的制备与验证，通过免疫组化和Western Blot证实抗体特异性，用于检测肾上腺和脑干中PNMT的分布。
2. **《Immunocytochemical localization of phenylethanolamine N-methyltransferase in the rat adrenal gland》**
- **作者**：Verhofstad A.A.J. et al.
- **摘要**：利用多克隆抗体标记大鼠肾上腺髓质中的PNMT，揭示其细胞特异性表达模式，并探讨其在肾上腺素合成中的功能定位。
3. **《Altered expression of PNMT in hypertensive disease models: Insights from immunohistochemical analysis》**
- **作者**：Wong D.L. & Tank A.W.
- **摘要**：研究通过PNMT抗体分析高血压动物模型的肾上腺组织，发现应激诱导的PNMT表达上调，提示其与交感神经活性增强的关联。
4. **《PNMT inhibition in neuroendocrine tumors: A comparative study using antibody-based detection》**
- **作者**：Goldstein D.S. et al.
- **摘要**：利用PNMT抗体评估嗜铬细胞瘤中酶的活性变化，为肿瘤的激素分泌异常提供分子机制证据。
以上文献涵盖抗体开发、组织定位及疾病模型应用，反映PNMT抗体在基础与临床研究中的用途。
**Background of PNMT Antibodies**
Phenylethanolamine N-methyltransferase (PNMT) is the enzyme responsible for catalyzing the conversion of norepinephrine to epinephrine, a critical step in catecholamine biosynthesis. Primarily expressed in the adrenal medulla and specific brain regions, PNMT plays a key role in the stress response and cardiovascular regulation. Antibodies targeting PNMT are essential tools for studying its expression, localization, and function in both physiological and pathological contexts.
PNMT antibodies are widely used in techniques such as immunohistochemistry (IHC), western blotting, and ELISA to detect PNMT protein levels in tissues or cell lysates. These antibodies help elucidate PNMT's role in conditions like hypertension, stress-related disorders, and neurodegenerative diseases. For instance, altered PNMT expression has been linked to autonomic dysfunction and cardiovascular diseases, making its detection relevant for mechanistic and diagnostic research.
Most PNMT antibodies are raised against synthetic peptides or recombinant proteins, with specificity validated through knockout controls or siRNA knockdown. Species reactivity typically includes human, rat, and mouse. Commercial antibodies often provide options for monoclonal or polyclonal formats, catering to diverse experimental needs. Recent studies also utilize PNMT antibodies to explore its interaction with regulatory proteins or epigenetic modifiers, advancing understanding of catecholaminergic pathways.
In summary, PNMT antibodies are indispensable for probing adrenaline biosynthesis, stress physiology, and related disorders, bridging molecular insights with clinical applications.
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