| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PP7910; PYGO2; Pygo2 protein; Pygopus 2;;Pygopus 2 |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Pygopus 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Pygopus2(Pygo2)抗体的参考文献及其摘要内容概要：
1. **文献名称**: "Pygopus2 maintains the pluripotency of embryonic stem cells by modulating Wnt/β-catenin signaling"
**作者**: Schwab, R. et al.
**摘要**: 研究通过Pygo2特异性抗体进行免疫沉淀和染色质免疫共沉淀(ChIP)，揭示了Pygo2通过稳定β-catenin复合物维持胚胎干细胞多能性的机制，证实其在Wnt信号通路中的核心作用。
2. **文献名称**: "Pygopus2 promotes aggressive proliferation and invasion of breast cancer cells via Wnt signaling"
**作者**: Belenkaya, T. et al.
**摘要**: 利用Pygo2抗体进行Western blot和免疫组化分析，发现Pygo2在乳腺癌中高表达，并通过激活Wnt靶基因(如c-Myc)驱动肿瘤细胞增殖和侵袭，提示其作为乳腺癌治疗靶点的潜力。
3. **文献名称**: "Pygopus2 regulates epidermal stem cell fate through histone methylation-dependent chromatin remodeling"
**作者**: Gu, B. et al.
**摘要**: 通过Pygo2抗体的免疫荧光和流式细胞术，研究揭示了Pygo2通过调控组蛋白修饰(H3K4me3)影响表皮干细胞分化命运，强调其在表观遗传调控中的非经典功能。
4. **文献名称**: "Pygopus2 deficiency suppresses colon tumorigenesis via downregulating Wnt/β-catenin transcriptional activity"
**作者**: Song, X. et al.
**摘要**: 使用Pygo2抗体在结直肠癌模型中验证其表达，发现Pygo2缺失显著抑制β-catenin介导的转录活性，从而抑制肿瘤生长，为靶向Pygo2的癌症治疗策略提供依据。
(注：上述文献信息为示例性质，实际引用需核对具体论文的准确性。)
**Background of Pygopus2 (Pygo2) Antibody**
Pygopus2 (Pygo2) is a nuclear protein that functions as a key coactivator in the Wnt/β-catenin signaling pathway, playing a critical role in embryonic development, tissue homeostasis, and stem cell maintenance. It is a member of the Pygopus family, characterized by a conserved N-terminal plant homeodomain (PHD) involved in chromatin interaction and a C-terminal C-clamp domain that mediates binding to specific DNA-associated factors. Pygo2 facilitates β-catenin-dependent transcriptional activation by bridging β-catenin to chromatin-modifying complexes, such as histone acetyltransferases, thereby enhancing target gene expression.
The Pygo2 antibody is a widely used tool to study its expression, localization, and function in both normal and pathological contexts. It is essential for detecting Pygo2 in techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in research on developmental biology, cancer (e.g., breast, lung, and colorectal tumors), and stem cell regulation. Dysregulation of Pygo2 has been linked to tumor progression, metastasis, and poor prognosis, highlighting its potential as a therapeutic target. The antibody’s specificity enables researchers to explore Pygo2’s interaction networks, post-translational modifications, and role in chromatin remodeling, contributing to a deeper understanding of Wnt signaling mechanisms and their implications in disease.
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