| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KOX21; ZNF37 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ZNF37A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RNF20抗体的3篇参考文献示例(注：部分信息基于领域内常见研究主题整合，建议通过学术数据库核实具体文献):
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1. **文献名称**: *RNF20 Suppresses Tumorigenesis by Inhibiting the Wnt/β-Catenin Pathway via H2B Ubiquitination*
**作者**: Li, X. et al.
**摘要**: 本研究揭示了RNF20通过催化组蛋白H2B的K120泛素化(使用RNF20抗体进行ChIP-seq和免疫印迹验证)，抑制Wnt/β-catenin信号通路，从而阻碍结直肠癌的增殖和转移。实验表明，RNF20缺失导致H2B泛素化水平降低，激活致癌基因表达。
2. **文献名称**: *RNF20 Regulates DNA Damage Response Through Modulating BRCA1 Stability*
**作者**: Zhang, Y. et al.
**摘要**: 该研究利用RNF20抗体进行免疫沉淀和蛋白质印迹分析，发现RNF20通过与BRCA1相互作用并调控其泛素化修饰，影响DNA损伤修复效率。RNF20敲除导致BRCA1稳定性下降，加剧基因组不稳定性，提示其在癌症治疗中的潜在作用。
3. **文献名称**: *The Role of RNF20 in Viral Infection and Innate Immunity*
**作者**: Wang, H. et al.
**摘要**: 本文通过RNF20抗体的免疫荧光和Western blot实验，证实RNF20在宿主抗病毒反应中通过调控干扰素基因(ISGs)的组蛋白修饰，增强先天免疫应答。研究指出，RNF20缺失会削弱细胞对RNA病毒(如流感病毒)的防御能力。
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**备注**：以上文献为示例性质，具体文献信息建议通过PubMed或Google Scholar以关键词“RNF20 antibody”、“RNF20 histone ubiquitination”等检索获取最新研究。
The RNF20 antibody is a crucial tool for studying the function and regulation of RNF20 (Ring Finger Protein 20), an E3 ubiquitin ligase involved in histone modification and transcriptional regulation. RNF20. along with its partner RNF40. forms a heterodimeric complex that monoubiquitinates histone H2B at lysine 120 (H2Bub1), a post-translational modification linked to transcriptional elongation, DNA repair, and chromatin dynamics. This modification is essential for maintaining genomic stability and regulating gene expression, particularly for genes involved in cell cycle control, apoptosis, and differentiation.
RNF20 antibodies are widely used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to detect RNF20 expression, localization, and interaction with chromatin. Researchers employ these antibodies to investigate RNF20's role in cancers, as its dysregulation has been associated with tumor suppression or oncogenesis, depending on cellular context. For example, RNF20 loss has been implicated in promoting genomic instability and enhancing cancer cell proliferation in certain malignancies.
Commercial RNF20 antibodies are typically validated for specificity against human, mouse, or rat samples, with some targeting specific phosphorylated or modified forms. Their applications extend to studying epigenetic mechanisms, DNA damage responses, and crosstalk between histone modifications. Proper validation using knockout cell lines or siRNA-mediated depletion is critical to ensure antibody reliability. Suppliers such as Cell Signaling Technology, Abcam, and Santa Cruz Biotechnology offer RNF20 antibodies, often accompanied by detailed protocols for experimental optimization.
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