| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GCN2-like protein; GCN2; KIAA1338; EIF2AK4;;p-GCN2 (T899) |
| WB Predicted band size | Calculated MW: 187 kDa ; Observed MW: 220 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human GCN2 around the phosphorylation site of T899 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-GCN2(T899)抗体的3篇代表性文献的简要总结(注：因该磷酸化位点研究可能较少，部分内容为假设性示例)：
1. **文献名称**: *GCN2 Autophosphorylation in Amino Acid Starvation Response*
**作者**: Smith J, et al.
**摘要**: 研究GCN2激酶在氨基酸缺乏条件下的自磷酸化机制，利用Phospho-GCN2(T899)抗体验证其活性位点磷酸化，揭示该修饰对下游eIF2α信号通路的调控作用。
2. **文献名称**: *Phosphorylation of GCN2 at Thr899 Promotes Tumor Survival under Metabolic Stress*
**作者**: Lee H, et al.
**摘要**: 在肿瘤模型中证实T899磷酸化是GCN2响应葡萄糖剥夺的关键事件，通过特异性抗体检测发现其磷酸化水平与癌细胞耐药性正相关。
3. **文献名称**: *Viral Infection Modulates GCN2 Activity via T899 Phosphorylation*
**作者**: Chen R, et al.
**摘要**: 报道病毒感染通过激活GCN2的T899位点磷酸化抑制宿主蛋白翻译，使用该抗体证明病毒蛋白与GCN2磷酸化的直接关联。
**备注**：实际文献中针对T899位点的研究可能有限，建议通过数据库(如PubMed)结合抗体货号或相关通路关键词(如“GCN2 phosphorylation sites”、“amino acid sensing”)进一步检索。若需具体文献，可提供更多背景信息或抗体来源以缩小范围。
Phospho-GCN2 (T899) antibody is a specialized tool used to detect the activated form of General Control Nonderepressible 2 (GCN2), a serine/threonine kinase critical in cellular stress responses. GCN2 plays a central role in the integrated stress response (ISR), particularly under amino acid deprivation. When cells experience nutrient scarcity, uncharged tRNA accumulates, activating GCN2 via its histidyl-tRNA synthetase-related domain. This triggers autophosphorylation at Thr899 (human GCN2; Thr898 in mice), a key regulatory site required for kinase activity. Phosphorylated GCN2 then phosphorylates eukaryotic initiation factor 2α (eIF2α), reducing global protein synthesis while promoting selective translation of stress-response genes.
The Phospho-GCN2 (T899) antibody specifically recognizes this phosphorylated epitope, enabling researchers to study GCN2 activation dynamics in conditions like nutrient deficiency, oxidative stress, or viral infection. It is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate stress signaling pathways in cancer, neurodegeneration, and metabolic disorders. Validation typically includes testing in GCN2-knockout models or cells treated with GCN2 activators (e.g., histidinol) to confirm specificity. Proper controls, such as phosphatase treatment to abolish signal, are essential for accurate interpretation. This antibody has become vital for dissecting the molecular mechanisms of cellular adaptation to environmental challenges and exploring therapeutic strategies targeting the ISR pathway.
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