首页 / 产品 / 抗体 / 一抗

Rabbit Monoclonal Aconitase1 Antibody

  • 中文名： Aconitase1抗体
  • 别    名： ACO 1; Aconitase; Citrate hydro lyase; IREB1; IRP 1; Iron responsive element binding protein 1;;Aconitase 1
货号: IPDX17035
Price： ￥1280
数量：
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesACO 1; Aconitase; Citrate hydro lyase; IREB1; IRP 1; Iron responsive element binding protein 1;;Aconitase 1
WB Predicted band size98 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Aconitase 1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

+ +

参考文献

以下是关于Aconitase 1(ACO1)抗体的3篇参考文献示例(内容基于典型研究领域虚构，供参考)：

1. **文献名称**：*"Aconitase 1 regulates cellular iron metabolism and promotes tumor growth in glioblastoma"*

**作者**：Smith J, et al.

**摘要**：研究利用ACO1抗体验证其在胶质母细胞瘤中的表达，发现ACO1通过调控铁代谢相关通路影响肿瘤细胞增殖，抗体特异性经Western blot和免疫组化确认。

2. **文献名称**：*"Antibody-based detection of ACO1 dysfunction in neurodegenerative diseases"*

**作者**：Lee H, et al.

**摘要**：通过ACO1抗体分析阿尔茨海默病模型中的线粒体功能异常，发现ACO1活性降低与氧化应激相关，抗体用于检测脑组织中的蛋白表达水平变化。

3. **文献名称**：*"Iron-responsive element binding by Aconitase 1: Role of antibody validation in mechanistic studies"*

**作者**：Garcia R, et al.

**摘要**：研究利用ACO1抗体探究其作为铁调节蛋白(IRP1)的双重功能，抗体特异性经siRNA敲除验证，揭示ACO1在铁稳态中的分子机制。

(注：以上文献为示例，实际引用需查询真实数据库如PubMed或Web of Science。)

背景信息

Aconitase 1 (ACO1), also known as iron regulatory protein 1 (IRP1), is a bifunctional mitochondrial enzyme involved in cellular iron homeostasis and energy metabolism. It catalyzes the isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle, relying on an iron-sulfur (4Fe-4S) cluster for enzymatic activity. Under low iron conditions, ACO1 loses this cluster and switches to its IRP1 form, binding to iron-responsive elements (IREs) in mRNAs to regulate iron uptake, storage, and utilization. Aconitase 1 antibodies are essential tools for studying its dual roles in metabolic regulation and iron sensing. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect ACO1 expression levels, subcellular localization, and tissue distribution. Researchers employ them to explore pathologies linked to ACO1 dysfunction, including neurodegenerative diseases (e.g., Alzheimer’s), cancer (where altered TCA cycle activity affects tumor growth), and iron-related disorders. Validated antibodies typically target conserved regions of ACO1 across species like humans, mice, and rats. Specificity is critical due to structural similarities with Aconitase 2 (ACO2), another isoform with distinct subcellular localization. Proper controls, such as knockout validation, are recommended to ensure accurate interpretation in experimental models.

客户数据及评论

折叠内容

大包装询价

×