| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KOX18; ZNF36; PHZ-37; ZNF139; ZSCAN33; 9130423L19Rik |
| WB Predicted band size | 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ZKSCAN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"Phosphorylation of protein phosphatase 2A at tyrosine 307 regulates interleukin-8 production by human mesenchymal stem cells"**
*Authors: Li X, et al.*
Summary：该研究探讨了PP2Aα在Y307位点的磷酸化如何通过调控NF-κB信号通路影响间充质干细胞IL-8的分泌，揭示了其在炎症反应中的作用，并利用Phospho-PP2Aalpha(Y307)抗体验证磷酸化水平与功能关联。
2. **"Tyrosine phosphorylation of protein phosphatase 2A in response to growth factors and oxidative stress"**
*Authors: Chen J, et al.*
Summary：通过Phospho-PP2Aalpha(Y307)抗体检测，发现生长因子(如EGF)和氧化应激可诱导PP2Aα Y307磷酸化，进而抑制其磷酸酶活性，促进Akt/mTOR通路激活，影响细胞存活与增殖。
3. **"PP2A phosphorylation at tyrosine 307 by Src kinase promotes tumor progression in colorectal cancer"**
*Authors: Wang Y, et al.*
Summary：研究证明Src激酶介导的PP2Aα Y307磷酸化抑制其抑癌功能，导致结直肠癌细胞增殖与转移。使用该抗体验证了临床样本中磷酸化水平与患者预后的相关性。
4. **"Dynamic regulation of PP2A phosphorylation and activity by upstream kinases in neuronal differentiation"**
*Authors: Smith R, et al.*
Summary：利用Phospho-PP2Aalpha(Y307)抗体发现，神经元分化过程中PP2Aα Y307磷酸化受ERK调控，其动态变化影响MAPK信号通路关闭，促进分化标志物表达。
注：以上文献名为示例性概括，实际引用需根据具体研究调整。建议通过PubMed/Google Scholar以关键词“PP2A Y307 phosphorylation”、“Phospho-PP2Aalpha(Y307) antibody”检索真实文献。
The Phospho-PP2Aalpha(Y307) antibody is a specialized tool used to detect the phosphorylation status of the alpha catalytic subunit of Protein Phosphatase 2A (PP2A) at tyrosine residue 307. PP2A is a highly conserved serine/threonine phosphatase critical for regulating diverse cellular processes, including cell cycle progression, signal transduction, and apoptosis. Its activity is tightly controlled by post-translational modifications, such as phosphorylation, and interactions with regulatory subunits.
Phosphorylation at Y307 in PP2Aalpha (encoded by PPP2CA) has been implicated in modulating PP2A’s enzymatic activity and substrate specificity. Studies suggest that phosphorylation at this site may alter PP2A’s conformation, potentially disrupting its binding to regulatory B subunits or influencing its association with other signaling complexes. This modification has been linked to pathological conditions, including cancer and neurodegenerative diseases, where dysregulated PP2A activity contributes to aberrant signaling.
The Phospho-PP2Aalpha(Y307) antibody enables researchers to investigate the dynamic regulation of PP2A in response to cellular stimuli or stress, providing insights into its role in health and disease. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess PP2A activation states in experimental models. Understanding Y307 phosphorylation may aid in developing therapeutic strategies targeting PP2A-related pathways.
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